BAIT

PRP19

PSO4, E3 ubiquitin-protein ligase PRP19, L000001506, YLL036C

Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain; relocalizes to the cytosol in response to hypoxia

UPS Project

GO Process (1)

GO Function (2)

GO Component (6)

Gene Ontology Biological Process

generation of catalytic spliceosome for first transesterification step [IMP]

Gene Ontology Molecular Function

first spliceosomal transesterification activity [IC]
ubiquitin-protein transferase activity [IDA, IMP]

Gene Ontology Cellular Component

Prp19 complex [IDA]

U2-type catalytic step 1 spliceosome [IDA]

cytoplasm [IDA]

cytosol [IDA]

mitochondrion [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

PRP45

FUN20, mRNA splicing protein PRP45, L000000638, YAL032C

Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene

GO Process (1)

GO Function (2)

GO Component (4)

Gene Ontology Biological Process

generation of catalytic spliceosome for second transesterification step [IGI, IMP]

Gene Ontology Molecular Function

first spliceosomal transesterification activity [IC]
second spliceosomal transesterification activity [IC]

Gene Ontology Cellular Component

U2-type catalytic step 1 spliceosome [IDA]

U2-type catalytic step 2 spliceosome [IDA]

nucleus [IDA]

post-mRNA release spliceosomal complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Co-purification

An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.

Publication

Composition and functional characterization of the yeast spliceosomal penta-snRNP.

Stevens SW, Ryan DE, Ge HY, Moore RE, Young MK, Lee TD, Abelson J

Pre-mRNA introns are spliced in a macromolecular machine, the spliceosome. For each round of splicing, the spliceosome assembles de novo in a series of ATP-dependent steps involving numerous changes in RNA-RNA and RNA-protein interactions. As currently understood, spliceosome assembly proceeds by addition of discrete U1, U2, and U4/U6*U5 snRNPs to a pre-mRNA substrate to form functional splicing complexes. We characterized ... [more]

Mol. Cell Jan. 01, 2002; 9(1);31-44 [Pubmed: 11804584]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PRP45 PRP19	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2002)	High	-	BioGRID	-
PRP45 PRP19	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2006)	High	-	BioGRID	-
PRP19 PRP45	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2006)	High	-	BioGRID	-
PRP45 PRP19	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	10	BioGRID	3603361
PRP19 PRP45	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Ohi MD (2002)	High	-	BioGRID	-
PRP45 PRP19	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.1837	BioGRID	1918761

Curated By

BioGRID

Interaction Summary

PRP19

Gene Ontology Biological Process

Gene Ontology Molecular Function

first spliceosomal transesterification activity [IC]ubiquitin-protein transferase activity [IDA, IMP]

Gene Ontology Cellular Component

PRP45

Gene Ontology Biological Process

Gene Ontology Molecular Function

first spliceosomal transesterification activity [IC]second spliceosomal transesterification activity [IC]

Gene Ontology Cellular Component

Co-purification

Publication

Composition and functional characterization of the yeast spliceosomal penta-snRNP.

Throughput

Related interactions

Curated By

first spliceosomal transesterification activity [IC]
ubiquitin-protein transferase activity [IDA, IMP]

first spliceosomal transesterification activity [IC]
second spliceosomal transesterification activity [IC]