BAIT

PRP45

FUN20, mRNA splicing protein PRP45, L000000638, YAL032C

Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene

GO Process (1)

GO Function (2)

GO Component (4)

Gene Ontology Biological Process

generation of catalytic spliceosome for second transesterification step [IGI, IMP]

Gene Ontology Molecular Function

first spliceosomal transesterification activity [IC]
second spliceosomal transesterification activity [IC]

Gene Ontology Cellular Component

U2-type catalytic step 1 spliceosome [IDA]

U2-type catalytic step 2 spliceosome [IDA]

nucleus [IDA]

post-mRNA release spliceosomal complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

PRP19

PSO4, E3 ubiquitin-protein ligase PRP19, L000001506, YLL036C

Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain; relocalizes to the cytosol in response to hypoxia

UPS Project

GO Process (1)

GO Function (2)

GO Component (6)

Gene Ontology Biological Process

generation of catalytic spliceosome for first transesterification step [IMP]

Gene Ontology Molecular Function

first spliceosomal transesterification activity [IC]
ubiquitin-protein transferase activity [IDA, IMP]

Gene Ontology Cellular Component

Prp19 complex [IDA]

U2-type catalytic step 1 spliceosome [IDA]

cytoplasm [IDA]

cytosol [IDA]

mitochondrion [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Proteome survey reveals modularity of the yeast cell machinery.

Gavin AC, Aloy P, Grandi P, Krause R, Boesche M, Marzioch M, Rau C, Jensen LJ, Bastuck S, Duempelfeld B, Edelmann A, Heurtier MA, Hoffman V, Hoefert C, Klein K, Hudak M, Michon AM, Schelder M, Schirle M, Remor M, Rudi T, Hooper S, Bauer A, Bouwmeester T, Casari G, Drewes G, Neubauer G, Rick JM, Kuster B, Bork P, Russell RB, Superti-Furga G

Protein complexes are key molecular entities that integrate multiple gene products to perform cellular functions. Here we report the first genome-wide screen for complexes in an organism, budding yeast, using affinity purification and mass spectrometry. Through systematic tagging of open reading frames (ORFs), the majority of complexes were purified several times, suggesting screen saturation. The richness of the data set ... [more]

Nature Mar. 30, 2006; 440(7084);631-6 [Pubmed: 16429126]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PRP45 PRP19	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2002)	High	-	BioGRID	-
PRP19 PRP45	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2006)	High	-	BioGRID	-
PRP45 PRP19	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	10	BioGRID	3603361
PRP19 PRP45	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Ohi MD (2002)	High	-	BioGRID	-
PRP19 PRP45	Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.	Stevens SW (2002)	Low	-	BioGRID	-
PRP45 PRP19	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.1837	BioGRID	1918761

Curated By

BioGRID

Interaction Summary

PRP45

Gene Ontology Biological Process

Gene Ontology Molecular Function

first spliceosomal transesterification activity [IC]second spliceosomal transesterification activity [IC]

Gene Ontology Cellular Component

PRP19

Gene Ontology Biological Process

Gene Ontology Molecular Function

first spliceosomal transesterification activity [IC]ubiquitin-protein transferase activity [IDA, IMP]

Gene Ontology Cellular Component

Affinity Capture-MS

Publication

Proteome survey reveals modularity of the yeast cell machinery.

Throughput

Related interactions

Curated By

first spliceosomal transesterification activity [IC]
second spliceosomal transesterification activity [IC]

first spliceosomal transesterification activity [IC]
ubiquitin-protein transferase activity [IDA, IMP]