ITGB4
Gene Ontology Biological Process
- amelogenesis [IMP]
- autophagy [IMP]
- cell adhesion [NAS]
- cell junction assembly [TAS]
- cell motility [IMP]
- cell-matrix adhesion [IMP]
- digestive tract development [IMP]
- extracellular matrix organization [TAS]
- hemidesmosome assembly [IDA, IMP, TAS]
- mesodermal cell differentiation [IEP]
- nail development [IMP]
- renal system development [IMP]
- response to wounding [IDA]
- skin development [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ALOX12
Gene Ontology Biological Process
- arachidonic acid metabolic process [IDA, ISS, TAS]
- cellular component movement [IMP]
- establishment of skin barrier [ISS]
- fatty acid oxidation [IMP]
- hepoxilin biosynthetic process [ISS]
- hepoxilin metabolic process [TAS]
- leukotriene A4 metabolic process [IDA]
- linoleic acid metabolic process [IDA]
- lipoxin A4 biosynthetic process [IDA]
- lipoxin B4 biosynthetic process [IDA]
- lipoxin metabolic process [TAS]
- lipoxygenase pathway [IDA, TAS]
- negative regulation of apoptotic process [IMP, ISS]
- negative regulation of muscle cell apoptotic process [IMP]
- negative regulation of platelet aggregation [ISS]
- positive regulation of angiogenesis [IMP]
- positive regulation of cell adhesion [IMP]
- positive regulation of cell growth [IMP]
- positive regulation of cell migration [IMP]
- positive regulation of cell proliferation [IMP, ISS]
- reactive oxygen species metabolic process [NAS]
- small molecule metabolic process [TAS]
- superoxide anion generation [NAS]
Gene Ontology Molecular Function- arachidonate 12-lipoxygenase activity [IDA, TAS]
- hepoxilin A3 synthase activity [TAS]
- hepoxilin-epoxide hydrolase activity [ISS]
- linoleate 13S-lipoxygenase activity [IDA]
- oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen [TAS]
- protein binding [IPI]
- arachidonate 12-lipoxygenase activity [IDA, TAS]
- hepoxilin A3 synthase activity [TAS]
- hepoxilin-epoxide hydrolase activity [ISS]
- linoleate 13S-lipoxygenase activity [IDA]
- oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen [TAS]
- protein binding [IPI]
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Convergence of eicosanoid and integrin biology: 12-lipoxygenase seeks a partner.
Integrins and enzymes of the eicosanoid pathway are both well-established contributors to cancer. However, this is the first report of the interdependence of the two signaling systems. In a screen for proteins that interacted with, and thereby potentially regulated, the human platelet-type 12-lipoxygenase (12-LOX, ALOX12), we identified the integrin β4 (ITGB4).Using a cultured mammalian cell model, we have demonstrated that ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ITGB4 ALOX12 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| ALOX12 ITGB4 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID