Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

ADP ribosylation factors 1 and 4 and group VIA phospholipase Aâ‚‚ regulate morphology and intraorganellar traffic in the endoplasmic reticulum-Golgi intermediate compartment.

Ben-Tekaya H, Kahn RA, Hauri HP

Organelle morphology of the endomembrane system is critical for optimal organelle function. ADP ribosylation factors (Arfs), a family of small GTPases, are required for maintaining the structure of the Golgi and endosomes. What determines the discontinuous nature of the endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC) as tubulovesicular clusters is unknown. In search of morphological determinants for the ERGIC, we found ... [more]

Mol. Biol. Cell Dec. 01, 2010; 21(23);4130-40 [Pubmed: 20881058]

Throughput

  • Low Throughput

Additional Notes

  • Figure 5

Curated By

  • BioGRID