PLA2G2A
Gene Ontology Biological Process
- defense response to Gram-positive bacterium [TAS]
- glycerophospholipid biosynthetic process [TAS]
- low-density lipoprotein particle remodeling [TAS]
- phosphatidic acid biosynthetic process [TAS]
- phosphatidic acid metabolic process [IDA]
- phosphatidylcholine acyl-chain remodeling [TAS]
- phosphatidylethanolamine acyl-chain remodeling [TAS]
- phosphatidylglycerol acyl-chain remodeling [TAS]
- phosphatidylinositol acyl-chain remodeling [TAS]
- phosphatidylserine acyl-chain remodeling [TAS]
- phospholipid metabolic process [IDA, TAS]
- positive regulation of inflammatory response [TAS]
- positive regulation of macrophage derived foam cell differentiation [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ALOX12
Gene Ontology Biological Process
- arachidonic acid metabolic process [IDA, ISS, TAS]
- cellular component movement [IMP]
- establishment of skin barrier [ISS]
- fatty acid oxidation [IMP]
- hepoxilin biosynthetic process [ISS]
- hepoxilin metabolic process [TAS]
- leukotriene A4 metabolic process [IDA]
- linoleic acid metabolic process [IDA]
- lipoxin A4 biosynthetic process [IDA]
- lipoxin B4 biosynthetic process [IDA]
- lipoxin metabolic process [TAS]
- lipoxygenase pathway [IDA, TAS]
- negative regulation of apoptotic process [IMP, ISS]
- negative regulation of muscle cell apoptotic process [IMP]
- negative regulation of platelet aggregation [ISS]
- positive regulation of angiogenesis [IMP]
- positive regulation of cell adhesion [IMP]
- positive regulation of cell growth [IMP]
- positive regulation of cell migration [IMP]
- positive regulation of cell proliferation [IMP, ISS]
- reactive oxygen species metabolic process [NAS]
- small molecule metabolic process [TAS]
- superoxide anion generation [NAS]
Gene Ontology Molecular Function- arachidonate 12-lipoxygenase activity [IDA, TAS]
- hepoxilin A3 synthase activity [TAS]
- hepoxilin-epoxide hydrolase activity [ISS]
- linoleate 13S-lipoxygenase activity [IDA]
- oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen [TAS]
- protein binding [IPI]
- arachidonate 12-lipoxygenase activity [IDA, TAS]
- hepoxilin A3 synthase activity [TAS]
- hepoxilin-epoxide hydrolase activity [ISS]
- linoleate 13S-lipoxygenase activity [IDA]
- oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen [TAS]
- protein binding [IPI]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Interactions of 12-lipoxygenase with phospholipase A2 isoforms following platelet activation through the glycoprotein VI collagen receptor.
Recent studies implicate the collagen receptor, glycoprotein VI (GPVI) in activation of platelet 12-lipoxygenase (p12-LOX). Herein, we show that GPVI-stimulated 12-hydro(peroxy)eicosatetraenoic acid (H(P)ETE) synthesis is inhibited by palmityl trifluromethyl ketone or oleyloxyethylphosphocholine , but not bromoenol lactone, implicating secretory and cytosolic, but not calcium-independent phospholipase A2 (PLA2) isoforms. Also, following GPVI activation, 12-LOX co-immunoprecipitates with both cytosolic and secretory PLA2 ... [more]
Throughput
- Low Throughput
Additional Notes
- Figure 2
Curated By
- BioGRID