AKAP5
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
TRPV4
Gene Ontology Biological Process
- actin cytoskeleton reorganization [ISS]
- actin filament organization [ISS]
- calcium ion import [ISS]
- calcium ion transmembrane transport [TAS]
- calcium ion transport [IDA, NAS]
- cell volume homeostasis [TAS]
- cell-cell junction assembly [ISS]
- cellular calcium ion homeostasis [IDA]
- cellular hypotonic response [ISS]
- cellular response to heat [ISS]
- cellular response to osmotic stress [ISS]
- cortical microtubule organization [ISS]
- ion transmembrane transport [TAS]
- microtubule polymerization [ISS]
- negative regulation of neuron projection development [ISS]
- osmosensory signaling pathway [ISS, TAS]
- positive regulation of cytosolic calcium ion concentration [IDA]
- positive regulation of microtubule depolymerization [ISS]
- response to mechanical stimulus [TAS]
- transmembrane transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Proinflammatory mediators modulate the heat-activated ion channel TRPV1 via the scaffolding protein AKAP79/150.
The ability of vertebrates to detect and avoid damaging extremes of temperature depends on activation of ion channels belonging to the thermo-TRP family. Injury or inflammation causes the release of inflammatory mediators which lower the threshold for detection of painful levels of heat, a process known as heat hyperalgesia. These inflammatory mediators act by at least three distinct intracellular signaling ... [more]
Throughput
- Low Throughput
Additional Notes
- Figure 2
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TRPV4 AKAP5 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 1526800 |
Curated By
- BioGRID