An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Proinflammatory mediators modulate the heat-activated ion channel TRPV1 via the scaffolding protein AKAP79/150.

Zhang X, Li L, McNaughton PA

The ability of vertebrates to detect and avoid damaging extremes of temperature depends on activation of ion channels belonging to the thermo-TRP family. Injury or inflammation causes the release of inflammatory mediators which lower the threshold for detection of painful levels of heat, a process known as heat hyperalgesia. These inflammatory mediators act by at least three distinct intracellular signaling ... [more]

Neuron Aug. 14, 2008; 59(3);450-61 [Pubmed: 18701070]

Throughput

Low Throughput

Additional Notes

Figure 2

Curated By

BioGRID

Interaction Summary

AKAP5

Gene Ontology Biological Process

Gene Ontology Molecular Function

adenylate cyclase binding [IPI]protein binding [IPI]protein kinase A binding [TAS]

Gene Ontology Cellular Component

TRPV3