BAIT

TFG2

transcription factor IIF subunit TFG2, L000002633, YGR005C

TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30

GO Process (5)

GO Function (2)

GO Component (1)

Gene Ontology Biological Process

RNA polymerase II transcriptional preinitiation complex assembly [IC]

promoter clearance from RNA polymerase II promoter [IC]

transcription elongation from RNA polymerase II promoter [IDA]

transcription initiation from RNA polymerase II promoter [IDA]

transcriptional start site selection at RNA polymerase II promoter [IMP]

Gene Ontology Molecular Function

core RNA polymerase II binding transcription factor activity [IDA]
core RNA polymerase II recruiting transcription factor activity [IC]

Gene Ontology Cellular Component

transcription factor TFIIF complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

SUA7

SOH4, TFIIB, L000002141, YPR086W

Transcription factor TFIIB; a general transcription factor required for transcription initiation and start site selection by RNA polymerase II

GO Process (4)

GO Function (4)

GO Component (2)

Gene Ontology Biological Process

RNA polymerase II transcriptional preinitiation complex assembly [IDA, IMP]

positive regulation of transcription regulatory region DNA binding [IDA]

transcriptional open complex formation at RNA polymerase II promoter [IMP]

transcriptional start site selection at RNA polymerase II promoter [IMP]

Gene Ontology Molecular Function

RNA polymerase II core binding [IDA, IPI]
TBP-class protein binding [IDA, IPI]
TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA, IMP]
core RNA polymerase II recruiting transcription factor activity [IDA, IMP]

Gene Ontology Cellular Component

nucleus [IDA]

transcriptional preinitiation complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Co-purification

An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.

Publication

A novel collection of accessory factors associated with yeast RNA polymerase II.

Wade PA, Werel W, Fentzke RC, Thompson NE, Leykam JF, Burgess RR, Jaehning JA, Burton ZF

A relatively simple subset of general transcription factors is sufficient for transcript initiation by RNA polymerase II. However, a recently identified "holoenzyme" contains additional accessory proteins required for mediating signals from some activators (Y-J. Kim et al., 1994, Cell 77, 599-608; A. Koleske and R. Young, 1994, Nature 368, 466-469). By immobilizing RNA polymerase II and associated proteins (RAPs) from ... [more]

Protein Expr. Purif. Aug. 01, 1996; 8(1);85-90 [Pubmed: 8812838]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SUA7 TFG2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	O'Brien MJ (2024)	High	-	BioGRID	-
TFG2 SUA7	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.5388	BioGRID	1934276

Curated By

BioGRID

Interaction Summary

TFG2

Gene Ontology Biological Process

Gene Ontology Molecular Function

core RNA polymerase II binding transcription factor activity [IDA]core RNA polymerase II recruiting transcription factor activity [IC]

Gene Ontology Cellular Component

SUA7

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core binding [IDA, IPI]TBP-class protein binding [IDA, IPI]TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA, IMP]core RNA polymerase II recruiting transcription factor activity [IDA, IMP]

Gene Ontology Cellular Component

Co-purification

Publication

A novel collection of accessory factors associated with yeast RNA polymerase II.

Throughput

Related interactions

Curated By

core RNA polymerase II binding transcription factor activity [IDA]
core RNA polymerase II recruiting transcription factor activity [IC]

RNA polymerase II core binding [IDA, IPI]
TBP-class protein binding [IDA, IPI]
TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA, IMP]
core RNA polymerase II recruiting transcription factor activity [IDA, IMP]