TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30

GO Process (5)

GO Function (2)

GO Component (1)

Gene Ontology Biological Process

RNA polymerase II transcriptional preinitiation complex assembly [IC]

promoter clearance from RNA polymerase II promoter [IC]

transcription elongation from RNA polymerase II promoter [IDA]

transcription initiation from RNA polymerase II promoter [IDA]

transcriptional start site selection at RNA polymerase II promoter [IMP]

Gene Ontology Molecular Function

core RNA polymerase II binding transcription factor activity [IDA]
core RNA polymerase II recruiting transcription factor activity [IC]

Gene Ontology Cellular Component

transcription factor TFIIF complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Protein interaction network revealed by quantitative proteomic analysis links TFIIB to multiple aspects of the transcription cycle.

O'Brien MJ, Ansari A

Although TFIIB is widely regarded as an initiation factor, recent reports have implicated it in multiple aspects of eukaryotic transcription. To investigate the broader role of TFIIB in transcription, we performed quantitative proteomic analysis of yeast TFIIB. We purified two different populations of TFIIB; one from soluble cell lysate, which is not engaged in transcription, and the other from the ... [more]

Biochim Biophys Acta Proteins Proteom Jan. 01, 2024; 1872(1);140968 [Pubmed: 37863410]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TFG2 SUA7	Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.	Wade PA (1996)	Low	-	BioGRID	-
TFG2 SUA7	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.5388	BioGRID	1934276

Curated By

BioGRID

Interaction Summary

SUA7

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core binding [IDA, IPI]TBP-class protein binding [IDA, IPI]TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA, IMP]core RNA polymerase II recruiting transcription factor activity [IDA, IMP]

Gene Ontology Cellular Component

TFG2

Gene Ontology Biological Process

Gene Ontology Molecular Function

core RNA polymerase II binding transcription factor activity [IDA]core RNA polymerase II recruiting transcription factor activity [IC]

Gene Ontology Cellular Component

Affinity Capture-MS

Publication

Protein interaction network revealed by quantitative proteomic analysis links TFIIB to multiple aspects of the transcription cycle.

Throughput

Related interactions

Curated By

RNA polymerase II core binding [IDA, IPI]
TBP-class protein binding [IDA, IPI]
TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA, IMP]
core RNA polymerase II recruiting transcription factor activity [IDA, IMP]

core RNA polymerase II binding transcription factor activity [IDA]
core RNA polymerase II recruiting transcription factor activity [IC]