BAIT

IL12B

CLMF, CLMF2, IL-12B, IMD28, IMD29, NKSF, NKSF2
interleukin 12B
GO Process (46)
GO Function (8)
GO Component (3)

Gene Ontology Biological Process

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Celecoxib inhibits interleukin-12 alphabeta and beta2 folding and secretion by a novel COX2-independent mechanism involving chaperones of the endoplasmic reticulum.

Alloza I, Baxter A, Chen Q, Matthiesen R, Vandenbroeck K

Celecoxib (CE) is a nonsteroidal anti-inflammatory drug (NSAID) that is a specific inhibitor of cyclooxygenase 2 (COX2). It is indicated for a variety of chronic inflammatory conditions, including rheumatoid arthritis. Over the last few years, adverse cardiovascular effects and increased risk for heart attacks have been associated with this drug. In addition, evidence is emerging for COX2-independent molecular targets. CE ... [more]

Mol. Pharmacol. May. 01, 2006; 69(5);1579-87 [Pubmed: 16467190]

Throughput

  • Low Throughput

Additional Notes

  • Figure 8

Curated By

  • BioGRID