BAIT

SWI5

DNA-binding transcription factor SWI5, L000002253, YDR146C

Transcription factor that recruits Mediator and Swi/Snf complexes; activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; required for expression of the HO gene controlling mating type switching; localization to nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase; SWI5 has a paralog, ACE2, that arose from the whole genome duplication

GO Process (3)

GO Function (4)

GO Component (2)

Gene Ontology Biological Process

positive regulation of mating type switching [IMP]

positive regulation of transcription on exit from mitosis, from RNA polymerase II promoter [IMP]

regulation of transcription involved in G1/S transition of mitotic cell cycle [IMP]

Gene Ontology Molecular Function

RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
mediator complex binding [IMP, IPI]
sequence-specific DNA binding [IDA]
sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity [IMP]

Gene Ontology Cellular Component

cytoplasm [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

CDC6

AAA family ATPase CDC6, L000000246, YJL194W

Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress

GO Process (4)

GO Function (7)

GO Component (4)

Gene Ontology Biological Process

G1/S transition of mitotic cell cycle [IMP]

pre-replicative complex assembly involved in nuclear cell cycle DNA replication [IDA, IMP]

regulation of DNA-dependent DNA replication initiation [IMP]

regulation of chromatin silencing at telomere [IMP]

Gene Ontology Molecular Function

ATP binding [IDA, IMP]
ATPase activity [IDA]
DNA replication origin binding [IDA]
GTP binding [IDA]
GTPase activity [IDA]
chromatin binding [IDA, IMP]
protein binding [IPI]

Gene Ontology Cellular Component

DNA replication preinitiation complex [IDA]

cytoplasm [IDA]

nuclear pre-replicative complex [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

Genetic and biochemical evaluation of the importance of Cdc6 in regulating mitotic exit.

Archambault V, Li CX, Tackett AJ, Wasch R, Chait BT, Rout MP, Cross FR

We evaluated the hypothesis that the N-terminal region of the replication control protein Cdc6 acts as an inhibitor of cyclin-dependent kinase (Cdk) activity, promoting mitotic exit. Cdc6 accumulation is restricted to the period from mid-cell cycle until the succeeding G1, due to proteolytic control that requires the Cdc6 N-terminal region. During late mitosis, Cdc6 is present at levels comparable with ... [more]

Mol. Biol. Cell Nov. 01, 2003; 14(11);4592-604 [Pubmed: 12960422]

Throughput

Low Throughput

Ontology Terms

phenotype: viability (APO:0000111)

Additional Notes

genetic complex
overexpression of Cdc6 can rescue the viability of a cdh1/swi5 double mutant

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CDC6 SWI5	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2010)	High	-0.1594	BioGRID	389361
CDC6 SWI5	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.2334	BioGRID	1993549
SWI5 CDC6	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.3429	BioGRID	2034617

Curated By

BioGRID

Interaction Summary

SWI5

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]mediator complex binding [IMP, IPI]sequence-specific DNA binding [IDA]sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity [IMP]

Gene Ontology Cellular Component

CDC6

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [IDA, IMP]ATPase activity [IDA]DNA replication origin binding [IDA]GTP binding [IDA]GTPase activity [IDA]chromatin binding [IDA, IMP]protein binding [IPI]

Gene Ontology Cellular Component

Dosage Rescue

Publication

Genetic and biochemical evaluation of the importance of Cdc6 in regulating mitotic exit.

Throughput

Ontology Terms

Additional Notes

Related interactions

Curated By

RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
mediator complex binding [IMP, IPI]
sequence-specific DNA binding [IDA]
sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity [IMP]

ATP binding [IDA, IMP]
ATPase activity [IDA]
DNA replication origin binding [IDA]
GTP binding [IDA]
GTPase activity [IDA]
chromatin binding [IDA, IMP]
protein binding [IPI]