BAIT
FAR1
cyclin-dependent protein serine/threonine kinase inhibiting protein FAR1, L000000600, YJL157C
CDK inhibitor and nuclear anchor; during the cell cycle Far1p sequesters the GEF Cdc24p in the nucleus; phosphorylation by Cdc28p-Cln results in SCFCdc4 complex-mediated ubiquitin-dependent degradation, releasing Cdc24p for export and activation of GTPase Cdc42p; in response to pheromone, phosphorylation of Far1p by MAPK Fus3p results in association with, and inhibition of Cdc28p-Cln, as well as Msn5p mediated nuclear export of Far1p-Cdc24p, targeting Cdc24p to polarity sites
GO Process (3)
GO Function (1)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
PREY
PEA2
DFG9, PPF2, L000003143, YER149C
Coiled-coil 12S polarisome subunit; required for polarity establishment, apical bud growth, shmoo formation, filamentous differentiation; involved in Bni1p localization at sites of polarized growth, controlling polarized assembly of actin cables; role in apical growth affects diploid-specific bipolar bud site selection; retains Slt2p at bud tip to regulate ER inheritance; role in Ca2+ influx, cell fusion; S288C allele encoding Leu409 rather than Met linked with non-invasion
GO Process (9)
GO Function (0)
GO Component (6)
Gene Ontology Biological Process
- bipolar cellular bud site selection [IMP]
- budding cell apical bud growth [IGI, IMP]
- establishment of cell polarity [IMP]
- filamentous growth [IMP]
- positive regulation of actin cytoskeleton reorganization [IMP]
- pseudohyphal growth [IMP]
- regulation of initiation of mating projection growth [IMP]
- regulation of protein localization [IMP]
- regulation of termination of mating projection growth [IMP]
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
The role of Far1p in linking the heterotrimeric G protein to polarity establishment proteins during yeast mating.
Heterotrimeric guanosine triphosphate (GTP)-binding proteins (G proteins) determine tissue and cell polarity in a variety of organisms. In yeast, cells orient polarized growth toward the mating partner along a pheromone gradient by a mechanism that requires Far1p and Cdc24p. Far1p bound Gbetagamma and interacted with polarity establishment proteins, which organize the actin cytoskeleton. Cells containing mutated Far1p unable to bind ... [more]
Science Nov. 20, 1998; 282(5393);1511-6 [Pubmed: 9822386]
Throughput
- Low Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Curated By
- BioGRID