SKP1
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [IMP]
- G2/M transition of mitotic cell cycle [IMP]
- SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [IDA]
- exit from mitosis [IMP]
- kinetochore assembly [IDA, IMP]
- protein complex assembly [IDA]
- protein neddylation [IGI, IMP]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IDA]
- regulation of exit from mitosis [IMP, IPI]
- regulation of protein complex assembly [IPI]
- septin ring assembly [IMP]
- vacuolar acidification [IGI, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
HSP82
Gene Ontology Biological Process
Gene Ontology Molecular Function
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
Hsp90 enables Ctf13p/Skp1p to nucleate the budding yeast kinetochore.
Binding of CBF3, a protein complex consisting of Ndc10p, Cep3p, Ctf13p, and Skp1p, to the centromere DNA nucleates kinetochore formation in budding yeast. Here, we investigate how the Ctf13p/Skp1p complex becomes competent to form the CBF3-centromere DNA complex. As revealed by mass spectrometry, Ctf13p and Skp1p carry two and four phosphate groups, respectively. Complete dephosphorylation of Ctf13p and Skp1p does ... [more]
Throughput
- Low Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Additional Notes
- deletion of SKP1 causes synthetic lethality when combined with an HSP82/HSC82 double mutant
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
HSP82 SKP1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
HSP82 SKP1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
HSP82 SKP1 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | High | - | BioGRID | 667097 | |
HSP82 SKP1 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | High | - | BioGRID | 306356 |
Curated By
- BioGRID