BAIT

AFT1

RCS1, DNA-binding transcription factor AFT1, L000002660, L000001594, YGL071W

Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions localization is regulated by Grx3p, Grx4p, and Fra2p, and promoter binding is negatively regulated via Grx3p-Grx4p binding; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress

GO Process (6)

GO Function (3)

GO Component (3)

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

AFT2

YPL202C

Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication

GO Process (4)

GO Function (3)

GO Component (2)

Gene Ontology Biological Process

cellular iron ion homeostasis [IGI]

cellular response to oxidative stress [IGI]

positive regulation of transcription from RNA polymerase II promoter [IDA]

positive regulation of transcription from RNA polymerase II promoter in response to iron ion starvation [IGI]

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IC]
sequence-specific DNA binding [IDA]

Gene Ontology Cellular Component

mitochondrion [IDA]

nucleus [IC]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

A second iron-regulatory system in yeast independent of Aft1p.

Rutherford JC, Jaron S, Ray E, Brown PO, Winge DR

Iron homeostasis in the yeast Saccharomyces cerevisiae is regulated at the transcriptional level by Aft1p, which activates the expression of its target genes in response to low-iron conditions. The yeast genome contains a paralog of AFT1, which has been designated AFT2. To establish whether AFT1 and AFT2 have overlapping functions, a mutant containing a double aft1Deltaaft2Delta deletion was generated. Growth ... [more]

Proc. Natl. Acad. Sci. U.S.A. Dec. 04, 2001; 98(25);14322-7 [Pubmed: 11734641]

Throughput

Low Throughput

Ontology Terms

phenotype: respiratory metabolism (APO:0000102)
phenotype: viability (APO:0000111)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
AFT2 AFT1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Bandyopadhyay S (2010)	High	-2.9391	BioGRID	541455
AFT1 AFT2	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.5209	BioGRID	2115045
AFT1 AFT2	Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.	Courel M (2005)	Low	-	BioGRID	164160
AFT1 AFT2	Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.	Portnoy ME (2002)	Low	-	BioGRID	156061
AFT1 AFT2	Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.	Rutherford JC (2001)	Low	-	BioGRID	161899
AFT1 AFT2	Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.	Blaiseau PL (2001)	Low	-	BioGRID	161800
AFT1 AFT2	Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.	Deepa A (2018)	Low	-	BioGRID	2454338
AFT1 AFT2	Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.	Dubacq C (2006)	Low	-	BioGRID	195849
AFT1 AFT2	Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.	Rutherford JC (2001)	Low	-	BioGRID	353984
AFT1 AFT2	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Berthelet S (2010)	High	-	BioGRID	442693

Curated By

BioGRID

Interaction Summary

AFT1

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity [IDA]
core promoter proximal region sequence-specific DNA binding [IDA]
sequence-specific DNA binding [IDA]

Gene Ontology Cellular Component

AFT2

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IC]
sequence-specific DNA binding [IDA]

Gene Ontology Cellular Component

Dosage Rescue

Publication

A second iron-regulatory system in yeast independent of Aft1p.

Throughput

Ontology Terms

Related interactions

Curated By

Interaction Summary

AFT1

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity [IDA]core promoter proximal region sequence-specific DNA binding [IDA]sequence-specific DNA binding [IDA]

Gene Ontology Cellular Component

AFT2

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IC]sequence-specific DNA binding [IDA]

Gene Ontology Cellular Component

Dosage Rescue

Publication

A second iron-regulatory system in yeast independent of Aft1p.

Throughput

Ontology Terms

Related interactions

Curated By

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity [IDA]
core promoter proximal region sequence-specific DNA binding [IDA]
sequence-specific DNA binding [IDA]

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IC]
sequence-specific DNA binding [IDA]