BAIT
SPF1
COD1, PER9, PIO1, ion-transporting P-type ATPase SPF1, L000003454, YEL031W
P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1
GO Process (4)
GO Function (1)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
TUP1
AAR1, AER2, AMM1, CRT4, CYC9, FLK1, ROX4, SFL2, UMR7, chromatin-silencing transcriptional regulator TUP1, L000002391, YCR084C
General repressor of transcription; forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes
GO Process (10)
GO Function (6)
GO Component (2)
Gene Ontology Biological Process
- histone exchange [IMP]
- negative regulation of dipeptide transport by negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of mating-type specific transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription from RNA polymerase II promoter by glucose [IMP]
- negative regulation of transcription from RNA polymerase II promoter during mitosis [IMP]
- nucleosome positioning [IDA]
- regulation of fatty acid biosynthetic process by regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of invasive growth in response to glucose limitation [IMP]
- regulation of response to DNA damage stimulus [IMP]
Gene Ontology Molecular Function- RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription [IDA, IMP]
- histone binding [IDA]
- histone deacetylase binding [IDA, IPI]
- mediator complex binding [IDA, IPI]
- phosphatidylinositol-3,5-bisphosphate binding [IDA]
- ubiquitin binding [IDA]
- RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription [IDA, IMP]
- histone binding [IDA]
- histone deacetylase binding [IDA, IPI]
- mediator complex binding [IDA, IPI]
- phosphatidylinositol-3,5-bisphosphate binding [IDA]
- ubiquitin binding [IDA]
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
Cooperative function of the CHD5-like protein Mdm39p with a P-type ATPase Spf1p in the maintenance of ER homeostasis in Saccharomyces cerevisiae.
Spf1p is a P-type ATPase that is mainly localized to the endoplasmic reticulum (ER) in Saccharomyces cerevisiae. The protein is involved in the maintenance of ion homeostasis in the ER. To investigate the intracellular role of Spf1p in more detail, we performed a genetic screen for mutations that lead to synthetic lethality in combination with a disruption of SPF1; the ... [more]
Mol. Genet. Genomics Jul. 01, 2005; 273(6);497-506 [Pubmed: 15909163]
Throughput
- Low Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Curated By
- BioGRID