SPT15
Gene Ontology Biological Process
Gene Ontology Molecular Function- DNA binding, bending [IDA]
- RNA polymerase I transcription factor binding [IDA, IPI]
- RNA polymerase I transcription factor recruiting transcription factor activity [IDA, IPI]
- RNA polymerase II activating transcription factor binding [IDA, IPI]
- RNA polymerase II core promoter sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IC]
- RNA polymerase III regulatory region DNA binding [IDA]
- TFIIIB-type transcription factor activity [IC]
- chromatin binding [IDA]
- sequence-specific DNA binding [IDA]
- DNA binding, bending [IDA]
- RNA polymerase I transcription factor binding [IDA, IPI]
- RNA polymerase I transcription factor recruiting transcription factor activity [IDA, IPI]
- RNA polymerase II activating transcription factor binding [IDA, IPI]
- RNA polymerase II core promoter sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IC]
- RNA polymerase III regulatory region DNA binding [IDA]
- TFIIIB-type transcription factor activity [IC]
- chromatin binding [IDA]
- sequence-specific DNA binding [IDA]
Gene Ontology Cellular Component
SUA7
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Dosage Rescue
A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.
Publication
The yeast FACT complex has a role in transcriptional initiation.
A crucial step in eukaryotic transcriptional initiation is recognition of the promoter TATA by the TATA-binding protein (TBP), which then allows TFIIA and TFIIB to be recruited. However, nucleosomes block the interaction between TBP and DNA. We show that the yeast FACT complex (yFACT) promotes TBP binding to a TATA box in chromatin both in vivo and in vitro. The ... [more]
Throughput
- Low Throughput
Ontology Terms
- phenotype: viability (APO:0000111)
Additional Notes
- TFIIB overexpression suppresses spt15, spt16 double mutant
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SUA7 SPT15 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| SUA7 SPT15 | Co-crystal Structure Co-crystal Structure Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex. | Low | - | BioGRID | - | |
| SPT15 SUA7 | Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps. | Low | - | BioGRID | - | |
| SUA7 SPT15 | Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene. | Low | - | BioGRID | 155568 | |
| SPT15 SUA7 | Far Western Far Western An interaction is detected between a protein immobilized on a membrane and a purified protein probe. | Low | - | BioGRID | - | |
| SUA7 SPT15 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| SUA7 SPT15 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| SPT15 SUA7 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| SPT15 SUA7 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| SPT15 SUA7 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| SPT15 SUA7 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 337316 | |
| SUA7 SPT15 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| SPT15 SUA7 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| SPT15 SUA7 | Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition. | Low | - | BioGRID | 160755 | |
| SPT15 SUA7 | Synthetic Rescue Synthetic Rescue A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene. | Low | - | BioGRID | 430026 | |
| SPT15 SUA7 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID