BAIT
MSS4
1-phosphatidylinositol-4-phosphate 5-kinase, L000001203, YDR208W
Phosphatidylinositol-4-phosphate 5-kinase; involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation
GO Process (2)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
MCK1
CMS1, YPK1, serine/threonine/tyrosine protein kinase MCK1, L000001036, S000029094, L000000369, YNL307C
Dual-specificity ser/thr and tyrosine protein kinase; roles in chromosome segregation, meiotic entry, genome stability, phosphorylation-dependent protein degradation (Rcn1p and Cdc6p), inhibition of protein kinase A, transcriptional regulation, inhibition of RNA pol III, calcium stress and inhibition of Clb2p-Cdc28p after nuclear division; MCK1 has a paralog, YGK3, that arose from the whole genome duplication
GO Process (9)
GO Function (5)
GO Component (0)
Gene Ontology Biological Process
- ascospore formation [IMP]
- cellular response to heat [IMP]
- cellular response to salt stress [IMP]
- double-strand break repair via nonhomologous end joining [IMP]
- meiotic nuclear division [IMP]
- mitotic sister chromatid segregation [IGI, IMP]
- peptidyl-serine phosphorylation [IDA]
- protein phosphorylation [IDA]
- protein phosphorylation involved in cellular protein catabolic process [IGI, IMP]
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
Genome-wide lethality screen identifies new PI4,5P2 effectors that regulate the actin cytoskeleton.
To further understand the roles played by the essential phosphoinositide PI4,5P(2), we have used a synthetic lethal analysis, which systematically combined the mss4(ts) mutation, partially defective in PI4P 5-kinase activity, with each of approximately 4700 deletion mutations. This genomic screening technique uncovered numerous new candidate effectors and regulators of PI4,5P(2) in yeast. In particular, we identified Slm1 (Yil105c), a previously ... [more]
EMBO J. Oct. 01, 2004; 23(19);3747-57 [Pubmed: 15372071]
Throughput
- Low Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Curated By
- BioGRID