BAIT
MAD3
L000003179, YJL013C
Subunit of spindle-assembly checkpoint complex; involved in delaying anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover; MAD3 has a paralog, BUB1, that arose from the whole genome duplication
GO Process (3)
GO Function (0)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
MCK1
CMS1, YPK1, serine/threonine/tyrosine protein kinase MCK1, L000001036, S000029094, L000000369, YNL307C
Dual-specificity ser/thr and tyrosine protein kinase; roles in chromosome segregation, meiotic entry, genome stability, phosphorylation-dependent protein degradation (Rcn1p and Cdc6p), inhibition of protein kinase A, transcriptional regulation, inhibition of RNA pol III, calcium stress and inhibition of Clb2p-Cdc28p after nuclear division; MCK1 has a paralog, YGK3, that arose from the whole genome duplication
GO Process (9)
GO Function (5)
GO Component (0)
Gene Ontology Biological Process
- ascospore formation [IMP]
- cellular response to heat [IMP]
- cellular response to salt stress [IMP]
- double-strand break repair via nonhomologous end joining [IMP]
- meiotic nuclear division [IMP]
- mitotic sister chromatid segregation [IGI, IMP]
- peptidyl-serine phosphorylation [IDA]
- protein phosphorylation [IDA]
- protein phosphorylation involved in cellular protein catabolic process [IGI, IMP]
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
Diverse functions of spindle assembly checkpoint genes in Saccharomyces cerevisiae.
The spindle assembly checkpoint regulates the metaphase-to-anaphase transition from yeast to humans. We examined the genetic interactions with four spindle assembly checkpoint genes to identify nonessential genes involved in chromosome segregation, to identify the individual roles of the spindle assembly checkpoint genes within the checkpoint, and to reveal potential complexity that may exist. We used synthetic genetic array (SGA) analysis ... [more]
Genetics Jan. 01, 2006; 172(1);53-65 [Pubmed: 16157669]
Throughput
- High Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Curated By
- BioGRID