Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.


[Screening of proteins interact with FMR1 by yeast two-hybrid system]

Chen Y, Sittler A, Yu M, Bardoni B, Wu G

OBJECTIVE: To establish yeast two-hybrid system for screening of protein(s) interacted with the fragile X metal retardation protein (FMRP). METHODS: Fragment of exon 11 to 15 of the FMR1 cDNA was recombined with DNA-binding domain of the pBTM116 vector as bait to screen a mouse embryo cDNA library. RESULTS: Thirteen clones were confirmed to be able to specifically interact with ... [more]

Zhongguo Yi Xue Ke Xue Yuan Xue Bao Jun. 01, 1998; 20(3);173-8 [Pubmed: 11367701]


  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.


Curated By

  • BioGRID