BAIT

GCD11

SUI4, translation initiation factor eIF2 subunit gamma, L000000677, YER025W

Gamma subunit of the translation initiation factor eIF2; involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met; mutations in human ortholog cause X-linked intellectual disability (XLID)

GO Process (3)

GO Function (5)

GO Component (4)

Gene Ontology Biological Process

formation of translation preinitiation complex [IDA]

positive regulation of translational fidelity [IMP]

translational initiation [IMP]

Gene Ontology Molecular Function

GTP binding [IMP]
protein binding [IPI]
tRNA binding [IMP]
translation initiation factor activity [IMP]
translation initiation factor binding [IDA]

Gene Ontology Cellular Component

eukaryotic 43S preinitiation complex [IDA]

eukaryotic 48S preinitiation complex [IDA]

eukaryotic translation initiation factor 2 complex [IMP]

multi-eIF complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

TIF5

SUI5, translation initiation factor eIF5, L000002305, YPR041W

Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2

GO Process (4)

GO Function (5)

GO Component (3)

Gene Ontology Biological Process

formation of cytoplasmic translation initiation complex [IDA]

mature ribosome assembly [IMP]

negative regulation of translational initiation [IGI, IMP]

regulation of translational initiation [IDA, IMP, IPI]

Gene Ontology Molecular Function

GDP-dissociation inhibitor activity [IDA, IMP]
GTPase activator activity [IPI, ISS]
protein binding [IPI]
translation initiation factor activity [IDA, ISS]
translation initiation factor binding [IDA, IPI]

Gene Ontology Cellular Component

cytosolic small ribosomal subunit [IPI]

eukaryotic 48S preinitiation complex [IDA]

multi-eIF complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Global landscape of protein complexes in the yeast Saccharomyces cerevisiae.

Krogan NJ, Cagney G, Yu H, Zhong G, Guo X, Ignatchenko A, Li J, Pu S, Datta N, Tikuisis AP, Punna T, Peregrin-Alvarez JM, Shales M, Zhang X, Davey M, Robinson MD, Paccanaro A, Bray JE, Sheung A, Beattie B, Richards DP, Canadien V, Lalev A, Mena F, Wong P, Starostine A, Canete MM, Vlasblom J, Wu S, Orsi C, Collins SR, Chandran S, Haw R, Rilstone JJ, Gandi K, Thompson NJ, Musso G, St Onge P, Ghanny S, Lam MH, Butland G, Altaf-Ul AM, Kanaya S, Shilatifard A, O'Shea E, Weissman JS, Ingles CJ, Hughes TR, Parkinson J, Gerstein M, Wodak SJ, Emili A, Greenblatt JF

Identification of protein-protein interactions often provides insight into protein function, and many cellular processes are performed by stable protein complexes. We used tandem affinity purification to process 4,562 different tagged proteins of the yeast Saccharomyces cerevisiae. Each preparation was analysed by both matrix-assisted laser desorption/ionization-time of flight mass spectrometry and liquid chromatography tandem mass spectrometry to increase coverage and accuracy. ... [more]

Nature Mar. 30, 2006; 440(7084);637-43 [Pubmed: 16554755]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TIF5 GCD11	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Hinnebusch AG (2004)	Low	-	BioGRID	-
GCD11 TIF5	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Krogan NJ (2004)	High	-	BioGRID	-
GCD11 TIF5	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	6	BioGRID	3617710
TIF5 GCD11	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Asano K (1999)	Low	-	BioGRID	-
TIF5 GCD11	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Asano K (2000)	Low	-	BioGRID	-
TIF5 GCD11	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Singh CR (2006)	Low	-	BioGRID	-
TIF5 GCD11	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Singh CR (2011)	Low	-	BioGRID	-
GCD11 TIF5	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Alone PV (2006)	Low	-	BioGRID	-
TIF5 GCD11	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Alone PV (2006)	Low	-	BioGRID	-
TIF5 GCD11	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Asano K (1999)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

GCD11

Gene Ontology Biological Process

Gene Ontology Molecular Function

GTP binding [IMP]protein binding [IPI]tRNA binding [IMP]translation initiation factor activity [IMP]translation initiation factor binding [IDA]

Gene Ontology Cellular Component

TIF5

Gene Ontology Biological Process

Gene Ontology Molecular Function

GDP-dissociation inhibitor activity [IDA, IMP]GTPase activator activity [IPI, ISS]protein binding [IPI]translation initiation factor activity [IDA, ISS]translation initiation factor binding [IDA, IPI]

Gene Ontology Cellular Component

Affinity Capture-MS

Publication

Global landscape of protein complexes in the yeast Saccharomyces cerevisiae.

Throughput

Related interactions

Curated By

GTP binding [IMP]
protein binding [IPI]
tRNA binding [IMP]
translation initiation factor activity [IMP]
translation initiation factor binding [IDA]

GDP-dissociation inhibitor activity [IDA, IMP]
GTPase activator activity [IPI, ISS]
protein binding [IPI]
translation initiation factor activity [IDA, ISS]
translation initiation factor binding [IDA, IPI]