CYP2E1
Gene Ontology Biological Process
- drug metabolic process [IDA, IMP]
- epoxygenase P450 pathway [IBA]
- exogenous drug catabolic process [IBA]
- heterocycle metabolic process [IDA]
- monoterpenoid metabolic process [IDA]
- oxidation-reduction process [IDA]
- small molecule metabolic process [TAS]
- steroid metabolic process [IMP]
- xenobiotic metabolic process [IBA, TAS]
Gene Ontology Molecular Function- arachidonic acid epoxygenase activity [IBA]
- enzyme binding [IPI]
- heme binding [IDA]
- monooxygenase activity [IDA]
- oxidoreductase activity [IDA]
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen [TAS]
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen [IBA]
- oxygen binding [TAS]
- steroid hydroxylase activity [IBA]
- arachidonic acid epoxygenase activity [IBA]
- enzyme binding [IPI]
- heme binding [IDA]
- monooxygenase activity [IDA]
- oxidoreductase activity [IDA]
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen [TAS]
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen [IBA]
- oxygen binding [TAS]
- steroid hydroxylase activity [IBA]
Gene Ontology Cellular Component
POR
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PCA
A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.
Publication
Bimolecular fluorescence complementation analysis of cytochrome p450 2c2, 2e1, and NADPH-cytochrome p450 reductase molecular interactions in living cells.
Interactions between cytochromes P450 (P450s) and P450 reductase are required for enzymatic activity, and homo- or heterooligomerization of P450s may also be functionally important. Bimolecular fluorescence complementation (BiFC) was used to examine P450 interactions in a natural membrane context within living cells. BiFC detects protein interactions in living cells by reconstitution of a fluorescent protein from two fragments that are ... [more]
Throughput
- Low Throughput
Additional Notes
- Bimolecular fluorescence complementation
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| POR CYP2E1 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| POR CYP2E1 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| CYP2E1 POR | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 833264 |
Curated By
- BioGRID