BAIT

TFB1

TFIIH/NER complex subunit TFB1, L000002284, YDR311W

Subunit of TFIIH and nucleotide excision repair factor 3 complexes; required for nucleotide excision repair, target for transcriptional activators; relocalizes to the cytosol in response to hypoxia

GO Process (4)

GO Function (3)

GO Component (5)

Gene Ontology Biological Process

nucleotide-excision repair [IMP]

phosphorylation of RNA polymerase II C-terminal domain [IDA]

transcription from RNA polymerase I promoter [IMP]

transcription from RNA polymerase II promoter [IDA]

Gene Ontology Molecular Function

core RNA polymerase binding transcription factor activity [IC]
phosphatidylinositol-3-phosphate binding [IDA]
phosphatidylinositol-5-phosphate binding [IDA]

Gene Ontology Cellular Component

core TFIIH complex [IDA]

cytosol [IDA]

holo TFIIH complex [IDA]

nucleotide-excision repair factor 3 complex [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

TFA1

L000002595, YKL028W

TFIIE large subunit; involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening

GO Process (2)

GO Function (3)

GO Component (3)

Gene Ontology Biological Process

transcription from RNA polymerase II promoter [IDA, IMP]

transcriptional open complex formation at RNA polymerase II promoter [IDA, IGI]

Gene Ontology Molecular Function

RNA polymerase II core binding [IDA, IPI]
TFIIH-class transcription factor binding [IDA]
single-stranded DNA binding [IDA]

Gene Ontology Cellular Component

mitochondrion [IDA]

transcription factor TFIIE complex [IDA]

transcriptional preinitiation complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Proteome survey reveals modularity of the yeast cell machinery.

Gavin AC, Aloy P, Grandi P, Krause R, Boesche M, Marzioch M, Rau C, Jensen LJ, Bastuck S, Duempelfeld B, Edelmann A, Heurtier MA, Hoffman V, Hoefert C, Klein K, Hudak M, Michon AM, Schelder M, Schirle M, Remor M, Rudi T, Hooper S, Bauer A, Bouwmeester T, Casari G, Drewes G, Neubauer G, Rick JM, Kuster B, Bork P, Russell RB, Superti-Furga G

Protein complexes are key molecular entities that integrate multiple gene products to perform cellular functions. Here we report the first genome-wide screen for complexes in an organism, budding yeast, using affinity purification and mass spectrometry. Through systematic tagging of open reading frames (ORFs), the majority of complexes were purified several times, suggesting screen saturation. The richness of the data set ... [more]

Nature Mar. 30, 2006; 440(7084);631-6 [Pubmed: 16429126]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TFB1 TFA1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2002)	High	-	BioGRID	-
TFA1 TFB1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	4	BioGRID	3607660
TFA1 TFB1	Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.	Murakami K (2012)	Low	-	BioGRID	-
TFA1 TFB1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.1381	BioGRID	1940757
TFA1 TFB1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Uetz P (2000)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

TFB1

Gene Ontology Biological Process

Gene Ontology Molecular Function

core RNA polymerase binding transcription factor activity [IC]phosphatidylinositol-3-phosphate binding [IDA]phosphatidylinositol-5-phosphate binding [IDA]

Gene Ontology Cellular Component

TFA1

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core binding [IDA, IPI]TFIIH-class transcription factor binding [IDA]single-stranded DNA binding [IDA]

Gene Ontology Cellular Component

Affinity Capture-MS

Publication

Proteome survey reveals modularity of the yeast cell machinery.

Throughput

Related interactions

Curated By

core RNA polymerase binding transcription factor activity [IC]
phosphatidylinositol-3-phosphate binding [IDA]
phosphatidylinositol-5-phosphate binding [IDA]

RNA polymerase II core binding [IDA, IPI]
TFIIH-class transcription factor binding [IDA]
single-stranded DNA binding [IDA]