RPB7
Gene Ontology Biological Process
- nuclear-transcribed mRNA catabolic process, exonucleolytic [IGI]
- positive regulation of nuclear-transcribed mRNA poly(A) tail shortening [IMP]
- positive regulation of translational initiation [IMP]
- transcription from RNA polymerase II promoter [IGI]
- transcription initiation from RNA polymerase II promoter [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
TFG1
Gene Ontology Biological Process
- RNA polymerase II transcriptional preinitiation complex assembly [IC]
- dephosphorylation of RNA polymerase II C-terminal domain [IDA, IGI]
- promoter clearance from RNA polymerase II promoter [IC]
- regulation of protein serine/threonine phosphatase activity [IDA]
- transcription elongation from RNA polymerase II promoter [IDA, IMP]
- transcription initiation from RNA polymerase II promoter [IDA, IMP]
- transcriptional start site selection at RNA polymerase II promoter [IGI, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Proteome survey reveals modularity of the yeast cell machinery.
Protein complexes are key molecular entities that integrate multiple gene products to perform cellular functions. Here we report the first genome-wide screen for complexes in an organism, budding yeast, using affinity purification and mass spectrometry. Through systematic tagging of open reading frames (ORFs), the majority of complexes were purified several times, suggesting screen saturation. The richness of the data set ... [more]
Throughput
- High Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RPB7 TFG1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| RPB7 TFG1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 6 | BioGRID | 3597739 | |
| TFG1 RPB7 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| TFG1 RPB7 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| TFG1 RPB7 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.1373 | BioGRID | 1935479 |
Curated By
- BioGRID