BAIT

TAF9

TAF17, TATA-binding protein-associated factor TAF9, TafII17, L000003459, YMR236W

Subunit (17 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3

GO Process (4)

GO Function (3)

GO Component (3)

Gene Ontology Biological Process

RNA polymerase II transcriptional preinitiation complex assembly [IMP]

chromatin modification [IDA]

histone acetylation [IDA]

transcription from RNA polymerase II promoter [IDA, IMP]

Gene Ontology Molecular Function

RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IC]
chromatin binding [IDA]
protein complex scaffold [IMP]

Gene Ontology Cellular Component

SAGA complex [IDA]

SLIK (SAGA-like) complex [IDA]

transcription factor TFIID complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

TAF4

MPT1, TAF48, TSG2, TafII48, L000001144, YMR005W

TFIID subunit (48 kDa); involved in RNA polymerase II transcription initiation; potential Cdc28p substrate

GO Process (2)

GO Function (3)

GO Component (1)

Gene Ontology Biological Process

RNA polymerase II transcriptional preinitiation complex assembly [IC]

transcription from RNA polymerase II promoter [IDA]

Gene Ontology Molecular Function

RNA polymerase II activating transcription factor binding [IDA, IPI]
RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IC]
chromatin binding [IDA]

Gene Ontology Cellular Component

transcription factor TFIID complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Proteome survey reveals modularity of the yeast cell machinery.

Gavin AC, Aloy P, Grandi P, Krause R, Boesche M, Marzioch M, Rau C, Jensen LJ, Bastuck S, Duempelfeld B, Edelmann A, Heurtier MA, Hoffman V, Hoefert C, Klein K, Hudak M, Michon AM, Schelder M, Schirle M, Remor M, Rudi T, Hooper S, Bauer A, Bouwmeester T, Casari G, Drewes G, Neubauer G, Rick JM, Kuster B, Bork P, Russell RB, Superti-Furga G

Protein complexes are key molecular entities that integrate multiple gene products to perform cellular functions. Here we report the first genome-wide screen for complexes in an organism, budding yeast, using affinity purification and mass spectrometry. Through systematic tagging of open reading frames (ORFs), the majority of complexes were purified several times, suggesting screen saturation. The richness of the data set ... [more]

Nature Mar. 30, 2006; 440(7084);631-6 [Pubmed: 16429126]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TAF4 TAF9	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2006)	High	-	BioGRID	-
TAF4 TAF9	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Kolesnikova O (2018)	High	-	BioGRID	-
TAF4 TAF9	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Krogan NJ (2006)	High	-	BioGRID	-
TAF9 TAF4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Lee KK (2011)	High	-	BioGRID	546783
TAF9 TAF4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	6	BioGRID	3617497
TAF9 TAF4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Sanders SL (2002)	High	-	BioGRID	-
TAF4 TAF9	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Sanders SL (2002)	High	-	BioGRID	-
TAF9 TAF4	Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.	Magtanong L (2011)	High	-	BioGRID	567395
TAF9 TAF4	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.4373	BioGRID	1947740
TAF9 TAF4	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Selleck W (2001)	Low	-	BioGRID	-
TAF4 TAF9	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Layer JH (2010)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

TAF9

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IC]chromatin binding [IDA]protein complex scaffold [IMP]

Gene Ontology Cellular Component

TAF4

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II activating transcription factor binding [IDA, IPI]RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IC]chromatin binding [IDA]

Gene Ontology Cellular Component

Affinity Capture-MS

Publication

Proteome survey reveals modularity of the yeast cell machinery.

Throughput

Related interactions

Curated By

RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IC]
chromatin binding [IDA]
protein complex scaffold [IMP]

RNA polymerase II activating transcription factor binding [IDA, IPI]
RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IC]
chromatin binding [IDA]