BAIT

YRA1

SHE11, RNA-binding protein YRA1, L000002959, L000002873, YDR381W

Nuclear polyadenylated RNA-binding protein; required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; interacts with DBP2; inhibits the helicase activity of Dbp2; functionally redundant with Yra2p, another REF family member

GO Process (3)

GO Function (2)

GO Component (1)

Gene Ontology Biological Process

mRNA export from nucleus [IGI]

negative regulation of ATP-dependent RNA helicase activity [IDA]

transcription-coupled nucleotide-excision repair [IMP]

Gene Ontology Molecular Function

ATP-dependent RNA helicase inhibitor activity [IDA]
RNA binding [IDA]

Gene Ontology Cellular Component

transcription export complex [IPI]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

SUB2

ATP-dependent RNA helicase SUB2, L000004574, YDL084W

Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress

GO Process (6)

GO Function (1)

GO Component (5)

Gene Ontology Biological Process

chromatin silencing at telomere [IMP]

mRNA 3'-end processing [IMP]

mRNA export from nucleus [IDA, IMP, IPI]

mRNA splicing, via spliceosome [IMP]

transcription elongation from RNA polymerase II promoter [IMP]

transcription-coupled nucleotide-excision repair [IMP]

Gene Ontology Molecular Function

ATP-dependent RNA helicase activity [IGI, ISA]

Gene Ontology Cellular Component

chromosome, telomeric region [IPI]

cytoplasm [IDA]

nucleus [IDA]

spliceosomal complex [TAS]

transcription export complex [IPI]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

A global genetic interaction network maps a wiring diagram of cellular function.

Costanzo M, VanderSluis B, Koch EN, Baryshnikova A, Pons C, Tan G, Wang W, Usaj M, Hanchard J, Lee SD, Pelechano V, Styles EB, Billmann M, van Leeuwen J, van Dyk N, Lin ZY, Kuzmin E, Nelson J, Piotrowski JS, Srikumar T, Bahr S, Chen Y, Deshpande R, Kurat CF, Li SC, Li Z, Usaj MM, Okada H, Pascoe N, San Luis BJ, Sharifpoor S, Shuteriqi E, Simpkins SW, Snider J, Suresh HG, Tan Y, Zhu H, Malod-Dognin N, Janjic V, Przulj N, Troyanskaya OG, Stagljar I, Xia T, Ohya Y, Gingras AC, Raught B, Boutros M, Steinmetz LM, Moore CL, Rosebrock AP, Caudy AA, Myers CL, Andrews B, Boone C

We generated a global genetic interaction network for Saccharomyces cerevisiae, constructing more than 23 million double mutants, identifying about 550,000 negative and about 350,000 positive genetic interactions. This comprehensive network maps genetic interactions for essential gene pairs, highlighting essential genes as densely connected hubs. Genetic interaction profiles enabled assembly of a hierarchical model of cell function, including modules corresponding to ... [more]

Science Sep. 23, 2016; 353(6306); [Pubmed: 27708008]

Quantitative Score

-0.5941 [SGA Score]

Throughput

High Throughput

Ontology Terms

phenotype: colony size (APO:0000063)

Additional Notes

Genetic interactions were considered significant if they had a p-value < 0.05 and an SGA score > 0.16 for positive interactions and SGA score < -0.12 for negative interactions.
alleles: yra1-5003 - sub2-1 [SGA score = -0.5941, P-value = 2.555E-32]

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
YRA1 SUB2	Affinity Capture-Luminescence Affinity Capture-Luminescence An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag.	Asada R (2023)	Low	-	BioGRID	-
SUB2 YRA1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Bonneau F (2023)	Low	-	BioGRID	-
YRA1 SUB2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Bonneau F (2023)	Low	-	BioGRID	-
YRA1 SUB2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Oeffinger M (2007)	High	-	BioGRID	-
YRA1 SUB2	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Johnson SA (2011)	Low	-	BioGRID	-
YRA1 SUB2	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Kim M (2019)	Low	-	BioGRID	-
SUB2 YRA1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Straesser K (2001)	Low	-	BioGRID	-
SUB2 YRA1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Straesser K (2002)	Low	-	BioGRID	-
SUB2 YRA1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Zenklusen D (2002)	Low	-	BioGRID	-
YRA1 SUB2	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Zenklusen D (2002)	Low	-	BioGRID	-
SUB2 YRA1	Co-crystal Structure Co-crystal Structure Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.	Ren Y (2017)	Low	-	BioGRID	-
YRA1 SUB2	Dosage Growth Defect Dosage Growth Defect A genetic interaction is inferred when over expression or increased dosage of one gene causes a growth defect in a strain that is mutated or deleted for another gene.	Gonzalez-Aguilera C (2008)	Low	-	BioGRID	329545
YRA1 SUB2	Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.	Kashyap AK (2005)	Low	-	BioGRID	163412
YRA1 SUB2	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Johnson SA (2011)	Low	-	BioGRID	-
SUB2 YRA1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Ren Y (2017)	Low	-	BioGRID	2200767
SUB2 YRA1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Straesser K (2001)	Low	-	BioGRID	-
YRA1 SUB2	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Zenklusen D (2002)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

YRA1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP-dependent RNA helicase inhibitor activity [IDA]RNA binding [IDA]

Gene Ontology Cellular Component

SUB2

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP-dependent RNA helicase activity [IGI, ISA]

Gene Ontology Cellular Component

Negative Genetic

Publication

A global genetic interaction network maps a wiring diagram of cellular function.

Quantitative Score

Throughput

Ontology Terms

Additional Notes

Related interactions

Curated By

ATP-dependent RNA helicase inhibitor activity [IDA]
RNA binding [IDA]