BAIT

CCL1

TFIIH complex kinase subunit CCL1, L000000237, YPR025C

Cyclin associated with protein kinase Kin28p; Kin28p is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters

Kinome Project (Sc)

GO Process (2)

GO Function (2)

GO Component (3)

Gene Ontology Biological Process

phosphorylation of RNA polymerase II C-terminal domain [IDA]

transcription from RNA polymerase II promoter [IDA]

Gene Ontology Molecular Function

core RNA polymerase binding transcription factor activity [IC]
cyclin-dependent protein serine/threonine kinase regulator activity [IGI, IPI]

Gene Ontology Cellular Component

TFIIK complex [IDA]

holo TFIIH complex [IDA]

mitochondrion [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

KIN28

TFIIH complex serine/threonine-protein kinase subunit KIN28, L000000905, YDL108W

Serine/threonine protein kinase, subunit of transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters; phosphorylates Ser5 residue of the PolII C-terminal domain (CTD) at gene promoters; relocalizes to the cytosol in response to hypoxia

Kinome Project (Sc)

GO Process (8)

GO Function (4)

GO Component (4)

Gene Ontology Biological Process

7-methylguanosine mRNA capping [IMP]

phosphorylation of RNA polymerase II C-terminal domain [IDA, IMP]

phosphorylation of RNA polymerase II C-terminal domain involved in recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex [IMP, IPI]

phosphorylation of RNA polymerase II C-terminal domain serine 5 residues involved in recruitment of mRNA capping enzyme to RNA polymerase II holoenzyme complex [IMP]

positive regulation of transcription from RNA polymerase II promoter [IDA]

protein phosphorylation [IDA]

transcription from RNA polymerase I promoter [IMP]

transcription from RNA polymerase II promoter [IDA, IMP]

Gene Ontology Molecular Function

RNA polymerase II carboxy-terminal domain kinase activity [IDA, IMP]
core RNA polymerase binding transcription factor activity [IC]
cyclin-dependent protein kinase activating kinase activity [IDA, IMP]
protein kinase activity [IDA]

Gene Ontology Cellular Component

TFIIK complex [IDA]

cytosol [IDA]

holo TFIIH complex [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

A global genetic interaction network maps a wiring diagram of cellular function.

Costanzo M, VanderSluis B, Koch EN, Baryshnikova A, Pons C, Tan G, Wang W, Usaj M, Hanchard J, Lee SD, Pelechano V, Styles EB, Billmann M, van Leeuwen J, van Dyk N, Lin ZY, Kuzmin E, Nelson J, Piotrowski JS, Srikumar T, Bahr S, Chen Y, Deshpande R, Kurat CF, Li SC, Li Z, Usaj MM, Okada H, Pascoe N, San Luis BJ, Sharifpoor S, Shuteriqi E, Simpkins SW, Snider J, Suresh HG, Tan Y, Zhu H, Malod-Dognin N, Janjic V, Przulj N, Troyanskaya OG, Stagljar I, Xia T, Ohya Y, Gingras AC, Raught B, Boutros M, Steinmetz LM, Moore CL, Rosebrock AP, Caudy AA, Myers CL, Andrews B, Boone C

We generated a global genetic interaction network for Saccharomyces cerevisiae, constructing more than 23 million double mutants, identifying about 550,000 negative and about 350,000 positive genetic interactions. This comprehensive network maps genetic interactions for essential gene pairs, highlighting essential genes as densely connected hubs. Genetic interaction profiles enabled assembly of a hierarchical model of cell function, including modules corresponding to ... [more]

Science Sep. 23, 2016; 353(6306); [Pubmed: 27708008]

Quantitative Score

-0.4156 [SGA Score]

Throughput

High Throughput

Ontology Terms

phenotype: colony size (APO:0000063)

Additional Notes

Genetic interactions were considered significant if they had a p-value < 0.05 and an SGA score > 0.16 for positive interactions and SGA score < -0.12 for negative interactions.
alleles: ccl1-ts4 - kin28-ts [SGA score = -0.4156, P-value = 2.353E-7]

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CCL1 KIN28	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Breitkreutz A (2010)	High	1	BioGRID	-
CCL1 KIN28	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2002)	High	-	BioGRID	-
CCL1 KIN28	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Krogan NJ (2006)	High	-	BioGRID	-
KIN28 CCL1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	10	BioGRID	3612475
KIN28 CCL1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Pflieger D (2008)	Low	-	BioGRID	-
CCL1 KIN28	Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.	Keogh MC (2002)	Low	-	BioGRID	-
CCL1 KIN28	Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.	Magtanong L (2011)	High	-	BioGRID	567252
KIN28 CCL1	Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.	Valay JG (1993)	Low	-	BioGRID	154151
KIN28 CCL1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.5044	BioGRID	1923758
CCL1 KIN28	Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.	Zhu J (2016)	Low	-	BioGRID	1870125
CCL1 KIN28	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Akhtar MS (2009)	Low	-	BioGRID	1112373
KIN28 CCL1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Hengartner CJ (1998)	Low	-	BioGRID	-
KIN28 CCL1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Luo J (2015)	Low	-	BioGRID	2346044
KIN28 CCL1	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Keogh MC (2002)	Low	-	BioGRID	158185
KIN28 CCL1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Feaver WJ (1997)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

CCL1

Gene Ontology Biological Process

Gene Ontology Molecular Function

core RNA polymerase binding transcription factor activity [IC]cyclin-dependent protein serine/threonine kinase regulator activity [IGI, IPI]

Gene Ontology Cellular Component

KIN28

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II carboxy-terminal domain kinase activity [IDA, IMP]core RNA polymerase binding transcription factor activity [IC]cyclin-dependent protein kinase activating kinase activity [IDA, IMP]protein kinase activity [IDA]

Gene Ontology Cellular Component

Negative Genetic

Publication

A global genetic interaction network maps a wiring diagram of cellular function.

Quantitative Score

Throughput

Ontology Terms

Additional Notes

Related interactions

Curated By

core RNA polymerase binding transcription factor activity [IC]
cyclin-dependent protein serine/threonine kinase regulator activity [IGI, IPI]

RNA polymerase II carboxy-terminal domain kinase activity [IDA, IMP]
core RNA polymerase binding transcription factor activity [IC]
cyclin-dependent protein kinase activating kinase activity [IDA, IMP]
protein kinase activity [IDA]