BAIT

SRB4

MED17, L000002051, YER022W

Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for basal RNA polymerase II transcription; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination

GO Process (2)

GO Function (3)

GO Component (1)

Gene Ontology Biological Process

positive regulation of transcription from RNA polymerase II promoter [IDA]

transcription initiation from RNA polymerase II promoter [IMP]

Gene Ontology Molecular Function

activating transcription factor binding [IDA]
core RNA polymerase II recruiting transcription factor activity [IMP, IPI]
structural molecule activity [IMP]

Gene Ontology Cellular Component

core mediator complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

MED8

L000003917, YBR193C

Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation

GO Process (2)

GO Function (4)

GO Component (1)

Gene Ontology Biological Process

negative regulation of transcription from RNA polymerase II promoter [IDA]

positive regulation of transcription from RNA polymerase II promoter [IMP]

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
RNA polymerase II transcription corepressor activity [IPI]
TBP-class protein binding [IDA]
structural molecule activity [IMP]

Gene Ontology Cellular Component

core mediator complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Co-purification

An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.

Publication

Head module control of mediator interactions.

Takagi Y, Calero G, Komori H, Brown JA, Ehrensberger AH, Hudmon A, Asturias F, Kornberg RD

Yeast Mediator proteins interacting with Med17(Srb4) have been expressed at a high level with the use of recombinant baculoviruses and recovered in homogeneous form as a seven subunit, 223 kDa complex. Electron microscopy and single-particle analysis identify this complex as the Mediator head module. The recombinant head module complements "headless" Mediator for the initiation of transcription in vitro. The module ... [more]

Mol. Cell Aug. 04, 2006; 23(3);355-64 [Pubmed: 16885025]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SRB4 MED8	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Chereji RV (2017)	High	-	BioGRID	-
MED8 SRB4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Eychenne T (2016)	Low	-	BioGRID	-
SRB4 MED8	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2006)	High	-	BioGRID	-
MED8 SRB4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Krogan NJ (2006)	High	-	BioGRID	-
SRB4 MED8	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Lariviere L (2008)	Low	-	BioGRID	-
MED8 SRB4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	10	BioGRID	3618875
SRB4 MED8	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Miller C (2012)	Low	-	BioGRID	-
SRB4 MED8	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Takagi Y (2006)	Low	-	BioGRID	-
SRB4 MED8	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Cai G (2010)	Low	-	BioGRID	-
MED8 SRB4	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Eyboulet F (2015)	Low	-	BioGRID	1518363
MED8 SRB4	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Kang JS (2001)	Low	-	BioGRID	-
SRB4 MED8	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Kang JS (2001)	Low	-	BioGRID	-
MED8 SRB4	Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.	Takagi Y (2006)	Low	-	BioGRID	-
SRB4 MED8	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.5577	BioGRID	1929511
SRB4 MED8	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Imasaki T (2011)	Low	-	BioGRID	553963
MED8 SRB4	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Osman S (2021)	Low	-	BioGRID	3563788
SRB4 MED8	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Guglielmi B (2004)	Low	-	BioGRID	-
SRB4 MED8	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Ito T (2001)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

SRB4

Gene Ontology Biological Process

Gene Ontology Molecular Function

activating transcription factor binding [IDA]core RNA polymerase II recruiting transcription factor activity [IMP, IPI]structural molecule activity [IMP]

Gene Ontology Cellular Component

MED8

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]RNA polymerase II transcription corepressor activity [IPI]TBP-class protein binding [IDA]structural molecule activity [IMP]

Gene Ontology Cellular Component

Co-purification

Publication

Head module control of mediator interactions.

Throughput

Related interactions

Curated By

activating transcription factor binding [IDA]
core RNA polymerase II recruiting transcription factor activity [IMP, IPI]
structural molecule activity [IMP]

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
RNA polymerase II transcription corepressor activity [IPI]
TBP-class protein binding [IDA]
structural molecule activity [IMP]