UBC4
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
RSP5
Gene Ontology Biological Process
- cellular response to UV [IMP]
- chromatin assembly or disassembly [IMP]
- late endosome to vacuole transport via multivesicular body sorting pathway [IMP, IPI]
- mitochondrion organization [IGI, IMP]
- positive regulation of endocytosis [IMP]
- positive regulation of fatty acid biosynthetic process [IMP]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IMP]
- positive regulation of receptor-mediated endocytosis [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IPI]
- protein autoubiquitination [IGI]
- protein monoubiquitination [IDA, IGI, IMP]
- protein polyubiquitination [IDA, IMP]
- protein ubiquitination [IDA]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IMP]
- regulation of actin cytoskeleton organization [IGI]
- regulation of dolichol biosynthetic process [IGI, IMP]
- regulation of ergosterol biosynthetic process [IGI, IMP]
- regulation of initiation of mating projection growth [IMP]
- regulation of mRNA export from nucleus [IMP, IPI]
- regulation of multivesicular body size [IMP]
- regulation of nitrogen utilization [IGI]
- regulation of phosphate metabolic process [IGI]
- regulation of protein localization [IMP, IPI]
- regulation of rRNA processing [IMP]
- regulation of ribosomal large subunit export from nucleus [IMP]
- regulation of tRNA export from nucleus [IMP]
- regulation of tRNA processing [IMP]
- regulation of ubiquinone biosynthetic process [IGI, IMP]
- response to drug [IMP, IPI]
- ribophagy [IGI]
- ubiquitin-dependent endocytosis [IMP]
- ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
A global genetic interaction network maps a wiring diagram of cellular function.
We generated a global genetic interaction network for Saccharomyces cerevisiae, constructing more than 23 million double mutants, identifying about 550,000 negative and about 350,000 positive genetic interactions. This comprehensive network maps genetic interactions for essential gene pairs, highlighting essential genes as densely connected hubs. Genetic interaction profiles enabled assembly of a hierarchical model of cell function, including modules corresponding to ... [more]
Quantitative Score
- -0.3332 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- Genetic interactions were considered significant if they had a p-value < 0.05 and an SGA score > 0.16 for positive interactions and SGA score < -0.12 for negative interactions.
- alleles: ubc4 - rsp5-1 [SGA score = -0.3116, P-value = 5.77E-5]
- alleles: ubc4 - rsp5-sm1 [SGA score = -0.3332, P-value = 4.414E-25]
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RSP5 UBC4 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | 1516765 | |
| RSP5 UBC4 | Co-crystal Structure Co-crystal Structure Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex. | Low | - | BioGRID | 677979 | |
| RSP5 UBC4 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.4095 | BioGRID | 1976612 | |
| RSP5 UBC4 | Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 349771 | |
| RSP5 UBC4 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| RSP5 UBC4 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 1035142 | |
| RSP5 UBC4 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 872418 | |
| RSP5 UBC4 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| RSP5 UBC4 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 1060087 | |
| RSP5 UBC4 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| RSP5 UBC4 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| RSP5 UBC4 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| RSP5 UBC4 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 904566 | |
| RSP5 UBC4 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| RSP5 UBC4 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 3015408 | |
| RSP5 UBC4 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 2935878 | |
| RSP5 UBC4 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 2900126 | |
| RSP5 UBC4 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 1513088 |
Curated By
- BioGRID