TWF1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ACT1
Gene Ontology Biological Process
- DNA repair [IDA]
- actomyosin contractile ring contraction [IDA, IMP]
- ascospore wall assembly [IDA]
- budding cell isotropic bud growth [TAS]
- cellular response to oxidative stress [IGI]
- chronological cell aging [IMP]
- endocytosis [IMP]
- establishment of cell polarity [IGI]
- establishment of mitotic spindle orientation [TAS]
- exocytosis [TAS]
- fungal-type cell wall organization [TAS]
- histone acetylation [IDA]
- mitochondrion inheritance [TAS]
- protein secretion [IGI, IMP]
- vacuole inheritance [IGI, IMP]
- vesicle transport along actin filament [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
A global genetic interaction network maps a wiring diagram of cellular function.
We generated a global genetic interaction network for Saccharomyces cerevisiae, constructing more than 23 million double mutants, identifying about 550,000 negative and about 350,000 positive genetic interactions. This comprehensive network maps genetic interactions for essential gene pairs, highlighting essential genes as densely connected hubs. Genetic interaction profiles enabled assembly of a hierarchical model of cell function, including modules corresponding to ... [more]
Quantitative Score
- -0.3428 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- Genetic interactions were considered significant if they had a p-value < 0.05 and an SGA score > 0.16 for positive interactions and SGA score < -0.12 for negative interactions.
- alleles: twf1 - act1-119 [SGA score = -0.3428, P-value = 1.569E-7]
- alleles: twf1 - act1-124 [SGA score = -0.1337, P-value = 1.882E-7]
- alleles: twf1 - act1-159 [SGA score = -0.2566, P-value = 0.002951]
- alleles: twf1 - act1-4 [SGA score = -0.1706, P-value = 0.005384]
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ACT1 TWF1 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | Low | - | BioGRID | - | |
| TWF1 ACT1 | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | Low | - | BioGRID | 145431 | |
| ACT1 TWF1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.5218 | BioGRID | 1979366 | |
| ACT1 TWF1 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| TWF1 ACT1 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| TWF1 ACT1 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| TWF1 ACT1 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 145430 |
Curated By
- BioGRID