BAIT

HFI1

ADA1, GAN1, SRM12, SUP110, L000003094, YPL254W

Adaptor protein required for structural integrity of the SAGA complex; a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions

GO Process (3)

GO Function (2)

GO Component (3)

Gene Ontology Biological Process

chromatin modification [IDA]

histone acetylation [IDA]

transcription from RNA polymerase II promoter [IMP]

Gene Ontology Molecular Function

transcription coactivator activity [IMP]
transcription cofactor activity [IMP, IPI]

Gene Ontology Cellular Component

Ada2/Gcn5/Ada3 transcription activator complex [IDA, IPI]

SAGA complex [IDA]

SLIK (SAGA-like) complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

TAF6

TAF60, TafII60, L000002828, YGL112C

Subunit (60 kDa) of TFIID and SAGA complexes; involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4; relocalizes to the cytosol in response to hypoxia

GO Process (4)

GO Function (4)

GO Component (5)

Gene Ontology Biological Process

RNA polymerase II transcriptional preinitiation complex assembly [IMP]

chromatin modification [IDA]

histone acetylation [IDA]

transcription from RNA polymerase II promoter [IDA]

Gene Ontology Molecular Function

RNA polymerase II activating transcription factor binding [IDA, IPI]
RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IC]
chromatin binding [IDA]
protein complex scaffold [IMP]

Gene Ontology Cellular Component

SAGA complex [IDA]

SLIK (SAGA-like) complex [IDA]

cytosol [IDA]

nucleus [IDA]

transcription factor TFIID complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

SAGA binds TBP via its Spt8 subunit in competition with DNA: implications for TBP recruitment.

Sermwittayawong D, Tan S

In yeast, the multisubunit SAGA (Spt-Ada-Gcn5-acetyltransferase) complex acts as a coactivator to recruit the TATA-binding protein (TBP) to the TATA box, a critical step in eukaryotic gene regulation. However, it is unclear which SAGA subunits are responsible for SAGA's direct interactions with TBP and precisely how SAGA recruits TBP to the promoter. We have used chemical crosslinking to identify Spt8 ... [more]

EMBO J. Aug. 23, 2006; 25(16);3791-800 [Pubmed: 16888622]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
HFI1 TAF6	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2006)	High	-	BioGRID	-
TAF6 HFI1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Ingvarsdottir K (2005)	High	-	BioGRID	-
HFI1 TAF6	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Kamata K (2016)	High	-	BioGRID	-
TAF6 HFI1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Krogan NJ (2006)	High	-	BioGRID	-
HFI1 TAF6	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Lee KK (2011)	High	-	BioGRID	545873
TAF6 HFI1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Lee KK (2011)	High	-	BioGRID	546708
TAF6 HFI1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	10	BioGRID	3617537
TAF6 HFI1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Rodriguez-Navarro S (2004)	High	-	BioGRID	-
TAF6 HFI1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Sanders SL (2002)	High	-	BioGRID	-
HFI1 TAF6	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Dewhurst-Maridor G (2017)	Low	-	BioGRID	-
HFI1 TAF6	Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.	Han Y (2014)	Low	-	BioGRID	1028915
TAF6 HFI1	Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.	Wang H (2020)	Low	-	BioGRID	2883113

Curated By

BioGRID

Interaction Summary

HFI1

Gene Ontology Biological Process

Gene Ontology Molecular Function

transcription coactivator activity [IMP]transcription cofactor activity [IMP, IPI]

Gene Ontology Cellular Component

TAF6

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II activating transcription factor binding [IDA, IPI]RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IC]chromatin binding [IDA]protein complex scaffold [IMP]

Gene Ontology Cellular Component

Affinity Capture-MS

Publication

SAGA binds TBP via its Spt8 subunit in competition with DNA: implications for TBP recruitment.

Throughput

Related interactions

Curated By

transcription coactivator activity [IMP]
transcription cofactor activity [IMP, IPI]

RNA polymerase II activating transcription factor binding [IDA, IPI]
RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IC]
chromatin binding [IDA]
protein complex scaffold [IMP]