BAIT

MRS3

L000001179, YJL133W
Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication
GO Process (2)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

YFH1

L000003525, YDL120W
Mitochondrial matrix iron chaperone; oxidizes and stores iron; interacts with Isu1p to promote Fe-S cluster assembly; mutation results in multiple Fe/S-dependent enzyme deficiencies; human frataxin homolog is mutated in Friedrich's ataxia
GO Process (3)
GO Function (3)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Mrs3p, Mrs4p, and frataxin provide iron for Fe-S cluster synthesis in mitochondria.

Zhang Y, Lyver ER, Knight SA, Pain D, Lesuisse E, Dancis A

Yeast Mrs3p and Mrs4p are evolutionarily conserved mitochondrial carrier proteins that transport iron into mitochondria under some conditions. Yeast frataxin (Yfh1p), the homolog of the human protein implicated in Friedreich ataxia, is involved in iron homeostasis. However, its precise functions are controversial. Anaerobically grown triple mutant cells (Deltamrs3/4/Deltayfh1) displayed a severe growth defect corrected by in vivo iron supplementation. Because ... [more]

J. Biol. Chem. Aug. 11, 2006; 281(32);22493-502 [Pubmed: 16769722]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: utilization of iron source (APO:0000157)
  • phenotype: anaerobic growth (APO:0000307)
  • phenotype: vegetative growth (APO:0000106)

Additional Notes

  • bypassed by iron supplementation
  • mrs3 mrs4 yfh1 triple mutant have severe growth defect
  • mrs3 mrs4 yfh1 triple mutant have severe growth defect bypassed by iron supplementation

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MRS3 YFH1
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
163862

Curated By

  • BioGRID