BAIT

RPB3

DNA-directed RNA polymerase II core subunit RPB3, B44, L000001677, L000001589, YIL021W
RNA polymerase II third largest subunit B44; part of central core; similar to prokaryotic alpha subunit
GO Process (2)
GO Function (2)
GO Component (2)
Saccharomyces cerevisiae (S288c)
PREY

ADR1

DNA-binding transcription factor ADR1, L000000050, YDR216W
Carbon source-responsive zinc-finger transcription factor; required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization
Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Subgenic Pol II interactomes identify region-specific transcription elongation regulators.

Harlen KM, Churchman LS

Transcription, RNA processing, and chromatin-related factors all interact with RNA polymerase II (Pol II) to ensure proper timing and coordination of transcription and co-transcriptional processes. Many transcription elongation regulators must function simultaneously to coordinate these processes, yet few strategies exist to explore the complement of factors regulating specific stages of transcription. To this end, we developed a strategy to purify ... [more]

Mol. Syst. Biol. Jan. 02, 2017; 13(1);900 [Pubmed: 28043953]

Throughput

  • High Throughput

Additional Notes

  • 3' PolII enriched factors
  • 5' PolII enriched factors

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ADR1 RPB3
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-4.8054BioGRID
508226

Curated By

  • BioGRID