Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The CUL3-KLHL18 ligase regulates mitotic entry and ubiquitylates Aurora-A.

Moghe S, Jiang F, Miura Y, Cerny RL, Tsai MY, Furukawa M

The cullin-RING family of ubiquitin ligases regulates diverse cellular functions, such as cell cycle control, via ubiquitylation of specific substrates. CUL3 targets its substrates through BTB proteins. Here we show that depletion of CUL3 and the BTB protein KLHL18 causes a delay in mitotic entry. Centrosomal activation of Aurora-A, a kinase whose activity is required for entry into mitosis, is ... [more]

Biol Open Feb. 15, 2012; 1(2);82-91 [Pubmed: 23213400]

Throughput

  • Low Throughput

Curated By

  • BioGRID