BAIT
CHEK1
CHK1
checkpoint kinase 1
GO Process (15)
GO Function (3)
GO Component (9)
Gene Ontology Biological Process
- DNA damage checkpoint [IDA, IMP]
- DNA damage induced protein phosphorylation [IDA]
- DNA repair [IMP]
- DNA replication [TAS]
- G2 DNA damage checkpoint [IMP]
- cellular response to DNA damage stimulus [IMP]
- cellular response to mechanical stimulus [IEP]
- chromatin-mediated maintenance of transcription [ISS]
- negative regulation of mitosis [IDA]
- peptidyl-threonine phosphorylation [IDA]
- regulation of double-strand break repair via homologous recombination [IDA]
- regulation of histone H3-K9 acetylation [ISS]
- regulation of mitotic centrosome separation [IDA]
- regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage [ISS]
- replicative senescence [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PIK3CA
CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha
phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha
GO Process (22)
GO Function (6)
GO Component (4)
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- T cell costimulation [TAS]
- T cell receptor signaling pathway [TAS]
- blood coagulation [TAS]
- cardiac muscle contraction [TAS]
- endothelial cell migration [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- innate immune response [TAS]
- insulin receptor signaling pathway [TAS]
- insulin receptor signaling pathway via phosphatidylinositol 3-kinase [TAS]
- leukocyte migration [TAS]
- negative regulation of anoikis [IMP]
- neurotrophin TRK receptor signaling pathway [TAS]
- phosphatidylinositol biosynthetic process [TAS]
- phosphatidylinositol phosphorylation [ISS]
- phosphatidylinositol-mediated signaling [TAS]
- phospholipid metabolic process [TAS]
- platelet activation [TAS]
- small molecule metabolic process [TAS]
- vasculature development [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Combinatorial CRISPR-Cas9 screens for de novo mapping of genetic interactions.
We developed a systematic approach to map human genetic networks by combinatorial CRISPR-Cas9 perturbations coupled to robust analysis of growth kinetics. We targeted all pairs of 73 cancer genes with dual guide RNAs in three cell lines, comprising 141,912 tests of interaction. Numerous therapeutically relevant interactions were identified, and these patterns replicated with combinatorial drugs at 75% precision. From these ... [more]
Nat. Methods Mar. 20, 2017; 0(); [Pubmed: 28319113]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
- phenotype: viability (PATO:0000169)
- phenotype: hela cell (BTO:0000567)
Additional Notes
- CRISPR GI screen
- Cell Line: HeLa EFO:0001185
- Experimental Setup: Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library: Dual-guide CRISPRn library
- Significance Threshold:FDR ~ 0.3
Curated By
- BioGRID