BAIT
MTOR
FRAP, FRAP1, FRAP2, RAFT1, RAPT1
mechanistic target of rapamycin (serine/threonine kinase)
GO Process (30)
GO Function (9)
GO Component (11)
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- T cell costimulation [TAS]
- TOR signaling [IMP]
- cell growth [IDA, TAS]
- cellular response to hypoxia [ISS]
- cellular response to nutrient levels [ISS]
- double-strand break repair via homologous recombination [IBA]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- growth [NAS]
- innate immune response [TAS]
- insulin receptor signaling pathway [TAS]
- negative regulation of autophagy [ISS]
- neurotrophin TRK receptor signaling pathway [TAS]
- peptidyl-serine phosphorylation [IMP]
- phosphatidylinositol-mediated signaling [TAS]
- phosphorylation [IDA]
- positive regulation of gene expression [IMP]
- positive regulation of lipid biosynthetic process [IMP]
- positive regulation of protein phosphorylation [IDA]
- positive regulation of transcription from RNA polymerase III promoter [IMP]
- positive regulation of translation [IDA]
- protein autophosphorylation [IDA]
- protein catabolic process [TAS]
- protein phosphorylation [IDA, IMP]
- regulation of actin cytoskeleton organization [IMP]
- response to amino acid [IDA]
- response to nutrient [NAS]
- response to stress [IMP]
- signal transduction [NAS]
Gene Ontology Molecular Function- RNA polymerase III type 1 promoter DNA binding [IDA]
- RNA polymerase III type 2 promoter DNA binding [IDA]
- RNA polymerase III type 3 promoter DNA binding [IDA]
- TFIIIC-class transcription factor binding [IDA]
- kinase activity [IDA, TAS]
- phosphoprotein binding [IPI]
- protein binding [IPI]
- protein dimerization activity [IBA]
- protein serine/threonine kinase activity [IDA, TAS]
- RNA polymerase III type 1 promoter DNA binding [IDA]
- RNA polymerase III type 2 promoter DNA binding [IDA]
- RNA polymerase III type 3 promoter DNA binding [IDA]
- TFIIIC-class transcription factor binding [IDA]
- kinase activity [IDA, TAS]
- phosphoprotein binding [IPI]
- protein binding [IPI]
- protein dimerization activity [IBA]
- protein serine/threonine kinase activity [IDA, TAS]
Gene Ontology Cellular Component
Homo sapiens
PREY
SMAD4
DPC4, JIP, MADH4, MYHRS
SMAD family member 4
GO Process (24)
GO Function (14)
GO Component (9)
Gene Ontology Biological Process
- BMP signaling pathway [IDA, TAS]
- SMAD protein complex assembly [IDA]
- SMAD protein signal transduction [IDA]
- gene expression [TAS]
- intracellular signal transduction [IDA]
- negative regulation of cell growth [IDA]
- negative regulation of protein catabolic process [IMP]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- negative regulation of transcription, DNA-templated [IDA]
- palate development [ISS]
- positive regulation of BMP signaling pathway [IMP]
- positive regulation of SMAD protein import into nucleus [ISS]
- positive regulation of epithelial to mesenchymal transition [ISS]
- positive regulation of pathway-restricted SMAD protein phosphorylation [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IDA, TAS]
- positive regulation of transcription, DNA-templated [IDA]
- positive regulation of transforming growth factor beta receptor signaling pathway [IDA]
- regulation of transforming growth factor beta receptor signaling pathway [IMP]
- regulation of transforming growth factor beta2 production [IMP]
- response to hypoxia [IMP]
- response to transforming growth factor beta [IDA]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
- transforming growth factor beta receptor signaling pathway [IDA, TAS]
Gene Ontology Molecular Function- DNA binding [IDA]
- I-SMAD binding [IPI]
- R-SMAD binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- core promoter proximal region sequence-specific DNA binding [IDA]
- identical protein binding [IPI]
- protein binding [IPI]
- protein binding transcription factor activity [IDA]
- protein homodimerization activity [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription regulatory region DNA binding [IDA]
- transforming growth factor beta receptor, common-partner cytoplasmic mediator activity [IDA]
- DNA binding [IDA]
- I-SMAD binding [IPI]
- R-SMAD binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- core promoter proximal region sequence-specific DNA binding [IDA]
- identical protein binding [IPI]
- protein binding [IPI]
- protein binding transcription factor activity [IDA]
- protein homodimerization activity [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription regulatory region DNA binding [IDA]
- transforming growth factor beta receptor, common-partner cytoplasmic mediator activity [IDA]
Gene Ontology Cellular Component
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Combinatorial CRISPR-Cas9 screens for de novo mapping of genetic interactions.
We developed a systematic approach to map human genetic networks by combinatorial CRISPR-Cas9 perturbations coupled to robust analysis of growth kinetics. We targeted all pairs of 73 cancer genes with dual guide RNAs in three cell lines, comprising 141,912 tests of interaction. Numerous therapeutically relevant interactions were identified, and these patterns replicated with combinatorial drugs at 75% precision. From these ... [more]
Nat. Methods Mar. 20, 2017; 0(); [Pubmed: 28319113]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
- phenotype: viability (PATO:0000169)
- phenotype: hela cell (BTO:0000567)
Additional Notes
- CRISPR GI screen
- Cell Line: HeLa EFO:0001185
- Experimental Setup: Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library: Dual-guide CRISPRn library
- Significance Threshold:FDR ~ 0.3
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SMAD4 MTOR | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - |
Curated By
- BioGRID