BAIT
RPS6KB1
PS6K, S6K, S6K-beta-1, S6K1, STK14A, p70 S6KA, p70(S6K)-alpha, p70-S6K, p70-alpha
ribosomal protein S6 kinase, 70kDa, polypeptide 1
GO Process (12)
GO Function (3)
GO Component (5)
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [IMP]
- TOR signaling [IDA]
- cellular response to growth factor stimulus [IDA]
- insulin receptor signaling pathway [TAS]
- negative regulation of apoptotic process [IMP]
- negative regulation of insulin receptor signaling pathway [IMP]
- phosphatidylinositol-mediated signaling [TAS]
- positive regulation of mitotic cell cycle [IMP]
- positive regulation of translation [IMP]
- positive regulation of translational initiation [IMP]
- protein phosphorylation [IDA]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
RRM2
R2, RR2, RR2M
ribonucleotide reductase M2
GO Process (7)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [TAS]
- deoxyribonucleotide biosynthetic process [ISS]
- mitotic cell cycle [TAS]
- nucleobase-containing small molecule interconversion [TAS]
- nucleobase-containing small molecule metabolic process [TAS]
- regulation of transcription involved in G1/S transition of mitotic cell cycle [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Combinatorial CRISPR-Cas9 screens for de novo mapping of genetic interactions.
We developed a systematic approach to map human genetic networks by combinatorial CRISPR-Cas9 perturbations coupled to robust analysis of growth kinetics. We targeted all pairs of 73 cancer genes with dual guide RNAs in three cell lines, comprising 141,912 tests of interaction. Numerous therapeutically relevant interactions were identified, and these patterns replicated with combinatorial drugs at 75% precision. From these ... [more]
Nat. Methods Mar. 20, 2017; 0(); [Pubmed: 28319113]
Throughput
- High Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507)
- phenotype: viability (PATO:0000169)
- phenotype: a-549 cell (BTO:0000018)
Additional Notes
- CRISPR GI screen
- Cell Line: A-549 EFO:0001086
- Experimental Setup: Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library: Dual-guide CRISPRn library
- Significance Threshold:FDR ~ 0.3
Curated By
- BioGRID