BAIT
IRS1
G972R, IRS-1
insulin receptor substrate 1
GO Process (28)
GO Function (10)
GO Component (9)
Gene Ontology Biological Process
- cellular response to insulin stimulus [IDA, ISO]
- insulin receptor signaling pathway [IBA, ISO]
- insulin secretion [IMP]
- insulin-like growth factor receptor signaling pathway [ISO]
- lipid catabolic process [IMP]
- mammary gland development [IGI]
- negative regulation of insulin receptor signaling pathway [ISO]
- negative regulation of insulin secretion [ISO]
- negative regulation of somatostatin secretion [ISO]
- phosphatidylinositol 3-kinase signaling [ISO]
- positive regulation of cell migration [IGI]
- positive regulation of fatty acid beta-oxidation [ISO]
- positive regulation of glucagon secretion [ISO]
- positive regulation of glucose import [ISO]
- positive regulation of glucose import in response to insulin stimulus [ISO]
- positive regulation of glucose metabolic process [ISO]
- positive regulation of glycogen biosynthetic process [ISO]
- positive regulation of insulin receptor signaling pathway [ISO]
- positive regulation of mesenchymal cell proliferation [IGI]
- positive regulation of phosphatidylinositol 3-kinase activity [IMP, ISO]
- positive regulation of phosphorylation [ISO]
- protein heterooligomerization [ISO]
- protein kinase B signaling [IGI]
- protein localization to nucleus [IDA, IMP]
- regulation of gene expression [IMP]
- response to caffeine [ISO]
- response to insulin [ISO]
- response to peptide hormone [ISO]
Gene Ontology Molecular Function- SH2 domain binding [ISO]
- insulin receptor binding [IBA, ISO]
- insulin-like growth factor receptor binding [ISO]
- phosphatidylinositol 3-kinase binding [IDA, ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- protein kinase C binding [ISO]
- protein kinase binding [IDA, ISO]
- signal transducer activity [IBA]
- transmembrane receptor protein tyrosine kinase adaptor activity [ISO]
- SH2 domain binding [ISO]
- insulin receptor binding [IBA, ISO]
- insulin-like growth factor receptor binding [ISO]
- phosphatidylinositol 3-kinase binding [IDA, ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- protein kinase C binding [ISO]
- protein kinase binding [IDA, ISO]
- signal transducer activity [IBA]
- transmembrane receptor protein tyrosine kinase adaptor activity [ISO]
Gene Ontology Cellular Component
Mus musculus
PREY
PTEN
2310035O07Rik, A130070J02Rik, AI463227, B430203M17Rik, MMAC1, TEP1
phosphatase and tensin homolog
GO Process (72)
GO Function (13)
GO Component (13)
Gene Ontology Biological Process
- activation of mitotic anaphase-promoting complex activity [IMP, ISO]
- angiogenesis [IMP]
- brain morphogenesis [IMP]
- canonical Wnt signaling pathway [ISO]
- cardiac muscle tissue development [IMP]
- cell migration [IMP]
- central nervous system development [IMP]
- central nervous system myelin maintenance [IMP]
- central nervous system neuron axonogenesis [IMP]
- dendritic spine morphogenesis [IMP]
- dentate gyrus development [IMP]
- dephosphorylation [IDA]
- endothelial cell migration [IMP]
- forebrain morphogenesis [IMP]
- heart development [IMP]
- inositol phosphate dephosphorylation [ISO]
- learning or memory [IMP]
- locomotor rhythm [IMP]
- locomotory behavior [IMP]
- long term synaptic depression [ISO]
- long-term synaptic potentiation [IMP]
- male mating behavior [IMP]
- maternal behavior [IMP]
- memory [ISO]
- multicellular organismal response to stress [IMP]
- negative regulation of G1/S transition of mitotic cell cycle [ISO]
- negative regulation of apoptotic process [IMP]
- negative regulation of axonogenesis [IMP]
- negative regulation of cell aging [IMP]
- negative regulation of cell migration [ISO]
- negative regulation of cell proliferation [IGI, IMP, ISO]
- negative regulation of cell size [IMP]
- negative regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle [ISO]
- negative regulation of dendritic spine morphogenesis [IMP]
- negative regulation of epithelial cell proliferation [IMP]
- negative regulation of excitatory postsynaptic membrane potential [IMP]
- negative regulation of focal adhesion assembly [ISO]
- negative regulation of myelination [IMP]
- negative regulation of organ growth [IMP]
- negative regulation of phagocytosis [ISO]
- negative regulation of protein kinase B signaling [IMP, ISO]
- negative regulation of protein phosphorylation [ISO]
- negative regulation of ribosome biogenesis [IMP]
- negative regulation of synaptic vesicle clustering [IMP]
- neuron projection development [ISO]
- neuron-neuron synaptic transmission [IMP]
- peptidyl-tyrosine dephosphorylation [ISO]
- phosphatidylinositol dephosphorylation [ISO]
- platelet-derived growth factor receptor signaling pathway [ISO]
- positive regulation of apoptotic process [ISO]
- positive regulation of apoptotic signaling pathway [IMP]
- positive regulation of cell proliferation [IMP]
- positive regulation of excitatory postsynaptic membrane potential [IMP]
- positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process [ISO]
- positive regulation of sequence-specific DNA binding transcription factor activity [ISO]
- postsynaptic density assembly [IMP]
- prepulse inhibition [IMP]
- presynaptic membrane assembly [IMP]
- prostate gland growth [IMP]
- protein dephosphorylation [ISO]
- protein kinase B signaling [IMP]
- protein stabilization [ISO]
- regulation of B cell apoptotic process [IMP]
- regulation of cell cycle [IGI]
- regulation of cellular component size [IMP]
- regulation of myeloid cell apoptotic process [IMP]
- regulation of neuron projection development [IMP]
- regulation of protein stability [ISO]
- rhythmic synaptic transmission [IMP]
- social behavior [IMP]
- synapse assembly [IMP]
- synapse maturation [IMP]
Gene Ontology Molecular Function- PDZ domain binding [ISO]
- anaphase-promoting complex binding [ISO]
- enzyme binding [ISO]
- inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity [ISO]
- phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity [IDA, ISO]
- phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity [ISO]
- phosphatidylinositol-3-phosphatase activity [ISO]
- phosphoprotein phosphatase activity [ISO]
- platelet-derived growth factor receptor binding [ISO]
- protein binding [IPI]
- protein kinase binding [IPI]
- protein serine/threonine phosphatase activity [ISO]
- protein tyrosine phosphatase activity [ISO]
- PDZ domain binding [ISO]
- anaphase-promoting complex binding [ISO]
- enzyme binding [ISO]
- inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity [ISO]
- phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity [IDA, ISO]
- phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity [ISO]
- phosphatidylinositol-3-phosphatase activity [ISO]
- phosphoprotein phosphatase activity [ISO]
- platelet-derived growth factor receptor binding [ISO]
- protein binding [IPI]
- protein kinase binding [IPI]
- protein serine/threonine phosphatase activity [ISO]
- protein tyrosine phosphatase activity [ISO]
Gene Ontology Cellular Component
- Schmidt-Lanterman incisure [IDA]
- cell projection [ISO]
- cytoplasm [IDA, ISO]
- cytoplasmic side of plasma membrane [ISO]
- dendritic spine [ISO]
- intracellular [IMP]
- mitochondrion [ISO]
- myelin sheath adaxonal region [IDA]
- neuron projection [IDA, ISO]
- nucleoplasm [ISO]
- nucleus [IDA, ISO]
- plasma membrane [ISO]
- postsynaptic membrane [ISO]
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Disruption of the Phosphate Transporter Pit1 in Hepatocytes Improves Glucose Metabolism and Insulin Signaling by Modulating the USP7/IRS1 Interaction.
The liver plays a central role in whole-body lipid and glucose homeostasis. Increasing dietary fat intake results in increased hepatic fat deposition, which is associated with a risk for development of insulin resistance and type 2 diabetes. In this study, we demonstrate a role for the phosphate inorganic transporter 1 (PiT1/SLC20A1) in regulating metabolism. Specific knockout of Pit1 in hepatocytes significantly improved ... [more]
Cell Rep Sep. 06, 2016; 16(10);2736-48 [Pubmed: 27568561]
Throughput
- Low Throughput
Curated By
- BioGRID