Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Derlin-1 promotes ubiquitylation and degradation of the epithelial Na(+) channel, ENaC.

You H, Ge Y, Zhang J, Cao Y, Xing J, Su D, Huang Y, Li M, Qu S, Sun F, Liang X

Ubiquitylation of the epithelial Na(+) channel (ENaC) plays a critical role in cellular functions, including transmembrane transport of Na(+), Na(+) and water balance, and blood pressure stabilization. Published studies have suggested that ENaC subunits are targets of ER-related degradation (ERAD) in yeast systems. However, the molecular mechanism underlying proteasome-mediated degradation of ENaC subunits remains to be established. Derlin-1, an E3 ... [more]

J. Cell. Sci. Mar. 15, 2017; 130(6);1027-1036 [Pubmed: 28137758]

Throughput

  • Low Throughput

Curated By

  • BioGRID