MDM2
Gene Ontology Biological Process
- DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [IMP, TAS]
- Fc-epsilon receptor signaling pathway [TAS]
- cellular response to hypoxia [IEP]
- epidermal growth factor receptor signaling pathway [TAS]
- establishment of protein localization [IDA]
- fibroblast growth factor receptor signaling pathway [TAS]
- innate immune response [TAS]
- negative regulation of DNA damage response, signal transduction by p53 class mediator [IDA]
- negative regulation of cell cycle arrest [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- peptidyl-lysine modification [IMP]
- phosphatidylinositol-mediated signaling [TAS]
- positive regulation of cell proliferation [TAS]
- positive regulation of mitotic cell cycle [IMP]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IDA]
- protein complex assembly [IDA]
- protein destabilization [IDA]
- protein localization to nucleus [IDA]
- protein ubiquitination [IDA]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IDA]
- regulation of protein catabolic process [IDA]
- response to antibiotic [IEP]
- synaptic transmission [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SIRT1
Gene Ontology Biological Process
- DNA repair [TAS]
- DNA replication [TAS]
- DNA synthesis involved in DNA repair [ISS]
- angiogenesis [IDA]
- cell aging [TAS]
- cellular glucose homeostasis [ISS]
- cellular response to DNA damage stimulus [IDA]
- cellular response to hydrogen peroxide [IDA]
- cellular response to hypoxia [IMP]
- cellular response to ionizing radiation [ISS]
- cellular response to starvation [ISS]
- cellular response to tumor necrosis factor [IDA]
- cellular triglyceride homeostasis [ISS]
- cholesterol homeostasis [ISS]
- chromatin organization [IMP]
- chromatin silencing [TAS]
- chromatin silencing at rDNA [IDA]
- circadian regulation of gene expression [IMP, ISS]
- establishment of chromatin silencing [IDA]
- fatty acid homeostasis [ISS]
- histone H3 deacetylation [IDA, IMP]
- histone H3-K9 modification [ISS]
- histone deacetylation [IDA]
- intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator [IMP]
- maintenance of chromatin silencing [IMP]
- methylation-dependent chromatin silencing [TAS]
- negative regulation of DNA damage response, signal transduction by p53 class mediator [IDA]
- negative regulation of I-kappaB kinase/NF-kappaB signaling [IDA]
- negative regulation of NF-kappaB transcription factor activity [IDA]
- negative regulation of TOR signaling [IMP]
- negative regulation of androgen receptor signaling pathway [IMP]
- negative regulation of apoptotic process [IMP]
- negative regulation of cAMP-dependent protein kinase activity [IDA]
- negative regulation of cell growth [IMP]
- negative regulation of cellular response to testosterone stimulus [IMP]
- negative regulation of cellular senescence [IDA]
- negative regulation of fat cell differentiation [ISS]
- negative regulation of helicase activity [IDA]
- negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator [ISS]
- negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway [IMP]
- negative regulation of peptidyl-lysine acetylation [IDA]
- negative regulation of phosphorylation [IMP]
- negative regulation of prostaglandin biosynthetic process [ISS]
- negative regulation of protein kinase B signaling [IMP]
- negative regulation of sequence-specific DNA binding transcription factor activity [IDA, IMP]
- negative regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- negative regulation of transcription, DNA-templated [IDA]
- negative regulation of transforming growth factor beta receptor signaling pathway [ISS]
- peptidyl-lysine acetylation [IMP]
- peptidyl-lysine deacetylation [IDA]
- positive regulation of DNA repair [IMP]
- positive regulation of MHC class II biosynthetic process [IDA]
- positive regulation of adaptive immune response [IDA]
- positive regulation of apoptotic process [IDA, IMP]
- positive regulation of cAMP-dependent protein kinase activity [IMP]
- positive regulation of cell proliferation [IMP]
- positive regulation of cellular senescence [IDA]
- positive regulation of cholesterol efflux [ISS]
- positive regulation of chromatin silencing [IMP]
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- positive regulation of histone H3-K9 methylation [IMP]
- positive regulation of insulin receptor signaling pathway [IDA]
- positive regulation of macroautophagy [IDA]
- positive regulation of macrophage apoptotic process [ISS]
- positive regulation of protein phosphorylation [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IMP]
- protein ADP-ribosylation [TAS]
- protein deacetylation [IDA, IMP]
- protein destabilization [ISS]
- protein ubiquitination [IDA]
- pyrimidine dimer repair by nucleotide-excision repair [IMP]
- regulation of bile acid biosynthetic process [ISS]
- regulation of cell proliferation [IMP]
- regulation of endodeoxyribonuclease activity [IMP]
- regulation of glucose metabolic process [ISS]
- regulation of mitotic cell cycle [IDA]
- regulation of peroxisome proliferator activated receptor signaling pathway [ISS]
- regulation of protein import into nucleus, translocation [IMP]
- regulation of smooth muscle cell apoptotic process [ISS]
- response to endoplasmic reticulum stress [ISS]
- response to hydrogen peroxide [IDA]
- response to insulin [ISS]
- response to oxidative stress [IDA]
- single strand break repair [IMP]
- triglyceride mobilization [ISS]
- white fat cell differentiation [ISS]
Gene Ontology Molecular Function- HLH domain binding [IPI]
- NAD+ ADP-ribosyltransferase activity [TAS]
- NAD-dependent histone deacetylase activity [IDA]
- NAD-dependent histone deacetylase activity (H3-K9 specific) [ISS]
- NAD-dependent protein deacetylase activity [IDA, IMP]
- bHLH transcription factor binding [IPI]
- deacetylase activity [IDA]
- enzyme binding [IPI]
- histone binding [IPI]
- histone deacetylase activity [IDA]
- identical protein binding [IPI]
- keratin filament binding [IPI]
- mitogen-activated protein kinase binding [IPI]
- p53 binding [IPI]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein deacetylase activity [IDA]
- transcription corepressor activity [IDA, ISS]
- transcription factor binding [IPI]
- HLH domain binding [IPI]
- NAD+ ADP-ribosyltransferase activity [TAS]
- NAD-dependent histone deacetylase activity [IDA]
- NAD-dependent histone deacetylase activity (H3-K9 specific) [ISS]
- NAD-dependent protein deacetylase activity [IDA, IMP]
- bHLH transcription factor binding [IPI]
- deacetylase activity [IDA]
- enzyme binding [IPI]
- histone binding [IPI]
- histone deacetylase activity [IDA]
- identical protein binding [IPI]
- keratin filament binding [IPI]
- mitogen-activated protein kinase binding [IPI]
- p53 binding [IPI]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein deacetylase activity [IDA]
- transcription corepressor activity [IDA, ISS]
- transcription factor binding [IPI]
Gene Ontology Cellular Component
- ESC/E(Z) complex [IDA]
- PML body [IDA]
- chromatin silencing complex [IDA]
- cytoplasm [IDA]
- mitochondrion [IDA]
- nuclear chromatin [IDA]
- nuclear envelope [IDA]
- nuclear euchromatin [IDA]
- nuclear heterochromatin [IDA]
- nuclear inner membrane [IDA]
- nucleolus [IDA]
- nucleoplasm [IDA]
- nucleus [IDA]
- rDNA heterochromatin [IDA]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Acetylation-dependent regulation of MDM2 E3 ligase activity dictates its oncogenic function.
Abnormal activation of the oncogenic E3 ubiquitin ligase murine double minute 2 (MDM2) is frequently observed in human cancers. By ubiquitinating the tumor suppressor p53 protein, which leads to its proteasome-mediated destruction, MDM2 limits the tumor-suppressing activity of p53. On the other hand, by ubiquitinating itself, MDM2 targets itself for destruction and promotes the p53 tumor suppressor pathway, a process ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SIRT1 MDM2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| SIRT1 MDM2 | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 2210560 |
Curated By
- BioGRID