BAIT

LSM4

SDB23, USS1, L000002447, YER112W
Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress
Saccharomyces cerevisiae (S288c)
PREY

MCA1

YCA1, Ca(2+)-dependent cysteine protease MCA1, YOR197W
Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics and lifespan extension; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that block potential dimerization
GO Process (2)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Yeast caspase 1 links messenger RNA stability to apoptosis in yeast.

Mazzoni C, Herker E, Palermo V, Jungwirth H, Eisenberg T, Madeo F, Falcone C

During the past years, yeasts have been successfully established as models to study the mechanisms of apoptotic regulation. We recently showed that mutations in the LSM4 gene, which is involved in messenger RNA decapping, lead to increased mRNA stability and apoptosis in yeast. Here, we show that mitochondrial function and YCA1, which encodes a budding yeast metacaspase, are necessary for ... [more]

EMBO Rep. Nov. 01, 2005; 6(11);1076-81 [Pubmed: 16170310]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: redox state (APO:0000218)
  • phenotype: apoptosis (APO:0000155)
  • phenotype: mitochondrial morphology (APO:0000055)
  • phenotype: nuclear morphology (APO:0000057)
  • phenotype: chromosome/plasmid maintenance (APO:0000143)

Additional Notes

  • Accumulation of reactive oxygen species is partially suppressed
  • DNA breakage is mostly suppressed
  • LSM4 was deleted and replaced with a truncated form of K. lactis LSM4

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
LSM4 MCA1
Synthetic Rescue
Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

Low-BioGRID
520174

Curated By

  • BioGRID