BAIT

RHEB

RHEB2
Ras homolog enriched in brain
Alzheimer's Disease Project
GO Process (4)
GO Function (3)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

HTT

HD, IT15
huntingtin
Alzheimer's Disease Project
Homo sapiens

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Huntingtin promotes mTORC1 signaling in the pathogenesis of Huntington's disease.

Pryor WM, Biagioli M, Shahani N, Swarnkar S, Huang WC, Page DT, MacDonald ME, Subramaniam S

In patients with Huntington's disease (HD), the protein huntingtin (Htt) has an expanded polyglutamine (poly-Q) tract. HD results in early loss of medium spiny neurons in the striatum, which impairs motor and cognitive functions. Identifying the physiological role and molecular functions of Htt may yield insight into HD pathogenesis. We found that Htt promotes signaling by mTORC1 [mechanistic target of ... [more]

Sci Signal Oct. 28, 2014; 7(349);ra103 [Pubmed: 25351248]

Throughput

  • Low Throughput

Additional Notes

  • GST pulldown

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HTT RHEB
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID