ARM
Gene Ontology Biological Process
- Wnt signaling pathway [IMP, ISS, NAS]
- branch fusion, open tracheal system [IMP]
- branching morphogenesis of an epithelial tube [IMP]
- cell adhesion [IMP]
- cell fate determination [IMP]
- cell morphogenesis [IMP]
- chitin-based larval cuticle pattern formation [IMP]
- compound eye morphogenesis [IMP]
- compound eye retinal cell programmed cell death [IMP]
- cuticle pattern formation [IMP]
- cytoskeletal anchoring at plasma membrane [IBA, ISS]
- delamination [IMP]
- dorsal closure [IGI, IMP]
- epithelial cell type specification, open tracheal system [IMP]
- epithelium development [IMP]
- establishment or maintenance of cell polarity [NAS]
- germarium-derived female germ-line cyst formation [IMP]
- heart development [IMP]
- heart formation [IMP]
- imaginal disc-derived wing expansion [IMP]
- long-term memory [IMP]
- negative regulation of heart induction by canonical Wnt signaling pathway [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IBA]
- nervous system development [IMP]
- neuroblast development [IMP]
- neuroblast fate commitment [IMP]
- neuroblast fate determination [NAS]
- oocyte development [IBA]
- oocyte localization involved in germarium-derived egg chamber formation [TAS]
- oogenesis [IMP]
- ovarian follicle cell development [TAS]
- photoreceptor cell differentiation [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription, DNA-templated [IMP]
- protein localization to adherens junction [IMP]
- protein localization to plasma membrane [IMP]
- regulation of cell shape [IMP]
- single organismal cell-cell adhesion [IMP]
- somatic stem cell maintenance [IMP]
- ventral furrow formation [IMP]
- wing disc morphogenesis [IMP]
- zonula adherens assembly [IMP]
Gene Ontology Molecular Function- RNA polymerase II transcription coactivator activity [IPI]
- alpha-catenin binding [IBA, NAS]
- cadherin binding [IBA, IMP]
- cytoskeletal protein binding [ISS]
- kinase binding [IPI]
- protein binding [IPI]
- protein complex binding [IPI]
- protein kinase binding [IBA]
- structural molecule activity [IBA]
- transcription coactivator activity [IDA]
- transcription factor binding [IDA]
- RNA polymerase II transcription coactivator activity [IPI]
- alpha-catenin binding [IBA, NAS]
- cadherin binding [IBA, IMP]
- cytoskeletal protein binding [ISS]
- kinase binding [IPI]
- protein binding [IPI]
- protein complex binding [IPI]
- protein kinase binding [IBA]
- structural molecule activity [IBA]
- transcription coactivator activity [IDA]
- transcription factor binding [IDA]
Gene Ontology Cellular Component
- Z disc [IBA]
- adherens junction [IDA, NAS]
- apical plasma membrane [IDA]
- apicolateral plasma membrane [TAS]
- axon [IDA]
- basolateral plasma membrane [IBA]
- beta-catenin destruction complex [IBA]
- catenin complex [IBA, IDA, ISS, NAS]
- cytoplasm [IDA, NAS]
- cytoplasmic side of plasma membrane [IBA]
- cytosol [IBA]
- desmosome [IBA]
- endocytic vesicle [IDA]
- fascia adherens [IBA]
- membrane [NAS]
- nucleus [IDA]
- plasma membrane [IDA]
- spot adherens junction [NAS]
- transcription factor complex [IBA]
- zonula adherens [IDA]
PAN
Gene Ontology Biological Process
- Wnt signaling pathway [NAS, TAS]
- canonical Wnt signaling pathway [IBA]
- embryonic pattern specification [IMP, ISS]
- heart development [IMP, TAS]
- imaginal disc-derived wing morphogenesis [IMP, ISS]
- mesodermal cell fate determination [TAS]
- negative regulation of Wnt signaling pathway [NAS]
- negative regulation of transcription, DNA-templated [IMP]
- positive regulation of gene expression [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IEP]
- positive regulation of transcription, DNA-templated [IMP]
- regulation of Wnt signaling pathway [IMP, ISS]
- regulation of hemocyte differentiation [IMP, ISS]
- regulation of stem cell proliferation [IMP]
- regulation of transcription from RNA polymerase II promoter [IBA]
- regulation of transcription, DNA-templated [ISS]
- salivary gland morphogenesis [IMP]
- segment polarity determination [NAS]
- spiracle morphogenesis, open tracheal system [IMP, ISS]
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA]
- beta-catenin binding [IBA]
- kinase binding [IPI]
- protein binding [IPI]
- repressing transcription factor binding [IPI]
- sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA]
- beta-catenin binding [IBA]
- kinase binding [IPI]
- protein binding [IPI]
- repressing transcription factor binding [IPI]
- sequence-specific DNA binding [IDA]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
An RNAi-based chemical genetic screen identifies three small-molecule inhibitors of the Wnt/wingless signaling pathway.
Misregulated β-catenin responsive transcription (CRT) has been implicated in the genesis of various malignancies, including colorectal carcinomas, and it is a key therapeutic target in combating various cancers. Despite significant effort, successful clinical implementation of CRT inhibitory therapeutics remains a challenging goal. This is, in part, because of the challenge of identifying inhibitory compounds that specifically modulate the nuclear transcriptional ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PAN ARM | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | FlyBase | - | |
| PAN ARM | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | FlyBase | - | |
| PAN ARM | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | FlyBase | - | |
| ARM PAN | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | FlyBase | - | |
| PAN ARM | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | FlyBase | - | |
| PAN ARM | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| ARM PAN | Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | FlyBase | - | |
| PAN ARM | Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | FlyBase | - | |
| ARM PAN | Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | FlyBase | - | |
| ARM PAN | Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | FlyBase | - | |
| ARM PAN | Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | FlyBase | - | |
| PAN ARM | Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | FlyBase | - | |
| ARM PAN | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - | |
| PAN ARM | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | FlyBase | - | |
| ARM PAN | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | FlyBase | - |