BRK
Gene Ontology Biological Process
- cell competition in a multicellular organism [IMP]
- imaginal disc-derived wing morphogenesis [IMP]
- negative regulation of gene expression [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA, IMP, TAS]
- negative regulation of transforming growth factor beta receptor signaling pathway [IMP, NAS]
- nervous system development [NAS]
- posterior Malpighian tubule development [IMP]
- regulation of BMP signaling pathway [TAS]
- regulation of gene expression [IMP]
- salivary gland boundary specification [NAS]
- transforming growth factor beta receptor signaling pathway [TAS]
- wing disc morphogenesis [IMP]
Gene Ontology Molecular Function
CTBP
Gene Ontology Biological Process
- Wnt signaling pathway [IGI]
- chaeta development [IMP]
- embryonic development via the syncytial blastoderm [IMP]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- negative regulation of transcription, DNA-templated [IDA, IMP]
- positive regulation of JAK-STAT cascade [IMP]
- regulation of Wnt signaling pathway [IMP]
- regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of transcription, DNA-templated [IMP]
- sensory organ precursor cell fate determination [IMP]
- wing disc development [IMP]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Brinker requires two corepressors for maximal and versatile repression in Dpp signalling.
decapentaplegic (dpp) encodes a Drosophila transforming growth factor-beta homologue that functions as a morphogen in the developing embryo and in adult appendage formation. In the wing imaginal disc, a Dpp gradient governs patterning along the anteroposterior axis by inducing regional expression of diverse genes in a concentration-dependent manner. Recent studies show that responses to graded Dpp activity also require an ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
BRK CTBP | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 2773599 | |
CTBP BRK | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - | |
BRK CTBP | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | FlyBase | - | |
BRK CTBP | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |