BRK
Gene Ontology Biological Process
- cell competition in a multicellular organism [IMP]
- imaginal disc-derived wing morphogenesis [IMP]
- negative regulation of gene expression [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA, IMP, TAS]
- negative regulation of transforming growth factor beta receptor signaling pathway [IMP, NAS]
- nervous system development [NAS]
- posterior Malpighian tubule development [IMP]
- regulation of BMP signaling pathway [TAS]
- regulation of gene expression [IMP]
- salivary gland boundary specification [NAS]
- transforming growth factor beta receptor signaling pathway [TAS]
- wing disc morphogenesis [IMP]
Gene Ontology Molecular Function
CTBP
Gene Ontology Biological Process
- Wnt signaling pathway [IGI]
- chaeta development [IMP]
- embryonic development via the syncytial blastoderm [IMP]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- negative regulation of transcription, DNA-templated [IDA, IMP]
- positive regulation of JAK-STAT cascade [IMP]
- regulation of Wnt signaling pathway [IMP]
- regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of transcription, DNA-templated [IMP]
- sensory organ precursor cell fate determination [IMP]
- wing disc development [IMP]
Gene Ontology Molecular Function
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
A Comprehensive Drosophila melanogaster Transcription Factor Interactome.
Combinatorial interactions among transcription factors (TFs) play essential roles in generating gene expression specificity and diversity in metazoans. Using yeast 2-hybrid (Y2H) assays on nearly all sequence-specific Drosophila TFs, we identified 1,983 protein-protein interactions (PPIs), more than doubling the number of currently known PPIs among Drosophila TFs. For quality assessment, we validated a subset of our interactions using MITOMI and ... [more]
Throughput
- Low Throughput
Additional Notes
- MITOMI
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
BRK CTBP | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | FlyBase | - | |
CTBP BRK | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - | |
BRK CTBP | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | FlyBase | - | |
BRK CTBP | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID