LOQS
Gene Ontology Biological Process
- central nervous system development [IMP]
- dosage compensation by hyperactivation of X chromosome [IGI]
- female germ-line stem cell asymmetric division [IMP]
- germ-line stem cell maintenance [IMP]
- miRNA metabolic process [IDA]
- pre-miRNA processing [IDA, IMP]
- production of siRNA involved in RNA interference [IDA, IMP]
- production of small RNA involved in gene silencing by RNA [IDA]
- regulation of production of small RNA involved in gene silencing by RNA [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
LOQS
Gene Ontology Biological Process
- central nervous system development [IMP]
- dosage compensation by hyperactivation of X chromosome [IGI]
- female germ-line stem cell asymmetric division [IMP]
- germ-line stem cell maintenance [IMP]
- miRNA metabolic process [IDA]
- pre-miRNA processing [IDA, IMP]
- production of siRNA involved in RNA interference [IDA, IMP]
- production of small RNA involved in gene silencing by RNA [IDA]
- regulation of production of small RNA involved in gene silencing by RNA [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Processing of Drosophila endo-siRNAs depends on a specific Loquacious isoform.
Drosophila melanogaster expresses three classes of small RNAs, which are classified according to their mechanisms of biogenesis. MicroRNAs are approximately 22-23 nucleotides (nt), ubiquitously expressed small RNAs that are sequentially processed from hairpin-like precursors by Drosha/Pasha and Dcr-1/Loquacious complexes. MicroRNAs usually associate with AGO1 and regulate the expression of protein-coding genes. Piwi-interacting RNAs (piRNAs) of approximately 24-28 nt associate with ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
LOQS LOQS | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | FlyBase | - | |
LOQS LOQS | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | Low | - | FlyBase | - | |
LOQS LOQS | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |