LOQS
Gene Ontology Biological Process
- central nervous system development [IMP]
- dosage compensation by hyperactivation of X chromosome [IGI]
- female germ-line stem cell asymmetric division [IMP]
- germ-line stem cell maintenance [IMP]
- miRNA metabolic process [IDA]
- pre-miRNA processing [IDA, IMP]
- production of siRNA involved in RNA interference [IDA, IMP]
- production of small RNA involved in gene silencing by RNA [IDA]
- regulation of production of small RNA involved in gene silencing by RNA [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
LOQS
Gene Ontology Biological Process
- central nervous system development [IMP]
- dosage compensation by hyperactivation of X chromosome [IGI]
- female germ-line stem cell asymmetric division [IMP]
- germ-line stem cell maintenance [IMP]
- miRNA metabolic process [IDA]
- pre-miRNA processing [IDA, IMP]
- production of siRNA involved in RNA interference [IDA, IMP]
- production of small RNA involved in gene silencing by RNA [IDA]
- regulation of production of small RNA involved in gene silencing by RNA [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
A protein interaction map of Drosophila melanogaster.
Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid-based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins and 20,405 interactions. A computational method of rating two-hybrid interaction confidence ... [more]
Throughput
- High Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
LOQS LOQS | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | FlyBase | - | |
LOQS LOQS | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | FlyBase | - | |
LOQS LOQS | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | Low | - | FlyBase | - |
Curated By
- BioGRID