ASRIJ
Gene Ontology Biological Process
Gene Ontology Cellular Component
ARF79F
Gene Ontology Biological Process
- ARF protein signal transduction [IGI]
- cell adhesion [IMP]
- compound eye development [IMP]
- endosomal transport [TAS]
- neurotransmitter secretion [TAS]
- positive regulation of endocytosis [IMP]
- post-Golgi vesicle-mediated transport [IMP]
- protein ADP-ribosylation [ISS, NAS]
- regulation of cell shape [IMP]
- regulation of hematopoietic stem cell differentiation [IMP]
- regulation of lipid storage [IDA]
- synaptic vesicle endocytosis [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
ARF1-GTP regulates Asrij to provide endocytic control of Drosophila blood cell homeostasis.
Drosophila melanogaster larval hematopoiesis is a well-established model to study mechanisms that regulate hematopoietic niche maintenance and control of blood cell precursor (prohemocyte) differentiation. Molecules that perturb niche function affect the balance between prohemocytes and differentiated hemocytes. The conserved hemocyte-specific endosomal protein Asrij is essential for niche function and prohemocyte maintenance. Elucidating how subcellular trafficking molecules can regulate signaling presents ... [more]
Throughput
- Low Throughput
Additional Notes
- Immunoprecipitation of Asrij and ARF1 from S2R+ protein extracts
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ARF79F ASRIJ | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | Low | - | FlyBase | - | |
| ASRIJ ARF79F | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |