ASRIJ
Gene Ontology Biological Process
Gene Ontology Cellular Component
ARF79F
Gene Ontology Biological Process
- ARF protein signal transduction [IGI]
- cell adhesion [IMP]
- compound eye development [IMP]
- endosomal transport [TAS]
- neurotransmitter secretion [TAS]
- positive regulation of endocytosis [IMP]
- post-Golgi vesicle-mediated transport [IMP]
- protein ADP-ribosylation [ISS, NAS]
- regulation of cell shape [IMP]
- regulation of hematopoietic stem cell differentiation [IMP]
- regulation of lipid storage [IDA]
- synaptic vesicle endocytosis [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
A protein interaction map of Drosophila melanogaster.
Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid-based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins and 20,405 interactions. A computational method of rating two-hybrid interaction confidence ... [more]
Throughput
- High Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
ASRIJ ARF79F | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | FlyBase | 2318741 | |
ARF79F ASRIJ | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | Low | - | FlyBase | - |
Curated By
- BioGRID