CAF1
Gene Ontology Biological Process
- DNA repair [TAS]
- DNA replication-dependent nucleosome assembly [NAS]
- chromatin assembly [IDA, ISS]
- chromatin silencing [IPI]
- dendrite morphogenesis [IMP]
- eggshell chorion gene amplification [IC]
- histone acetylation [IDA, ISS]
- histone methylation [IDA]
- mitotic cytokinesis [IMP]
- muscle organ development [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- neuron development [IMP]
- neuron projection morphogenesis [IMP]
- nucleosome assembly [IDA, NAS]
- nucleosome mobilization [IDA]
- nucleosome positioning [IDA]
- positive regulation of cell proliferation [IMP]
- regulation of histone H3-K27 methylation [IMP]
- regulation of mitotic cell cycle [IMP]
- segment specification [IMP]
- transcription, DNA-templated [IDA]
Gene Ontology Molecular Function- chromatin binding [NAS]
- histone acetyltransferase binding [IPI, ISS]
- histone binding [IDA, NAS, TAS]
- histone deacetylase binding [IDA, ISS]
- histone methyltransferase activity [IDA]
- histone methyltransferase activity (H3-K27 specific) [IC]
- histone methyltransferase activity (H3-K9 specific) [IC]
- nucleosome binding [IDA]
- protein binding [IPI]
- protein homodimerization activity [IPI]
- chromatin binding [NAS]
- histone acetyltransferase binding [IPI, ISS]
- histone binding [IDA, NAS, TAS]
- histone deacetylase binding [IDA, ISS]
- histone methyltransferase activity [IDA]
- histone methyltransferase activity (H3-K27 specific) [IC]
- histone methyltransferase activity (H3-K9 specific) [IC]
- nucleosome binding [IDA]
- protein binding [IPI]
- protein homodimerization activity [IPI]
Gene Ontology Cellular Component
MBD-LIKE
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The Nucleosome Remodeling and Deacetylase Complex NuRD Is Built from Preformed Catalytically Active Sub-modules.
The nucleosome remodeling deacetylase (NuRD) complex is a highly conserved regulator of chromatin structure and transcription. Structural studies have shed light on this and other chromatin modifying machines, but much less is known about how they assemble and whether stable and functional sub-modules exist that retain enzymatic activity. Purification of the endogenous Drosophila NuRD complex shows that it consists of ... [more]
Throughput
- High Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CAF1 MBD-LIKE | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| MBD-LIKE CAF1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| MBD-LIKE CAF1 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | Low | - | FlyBase | - | |
| MBD-LIKE CAF1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| MBD-LIKE CAF1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| CAF1 MBD-LIKE | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |