BAIT

LEM2

heh1, SPAC18G6.10
LEM domain protein Heh1/Lem2
Schizosaccharomyces pombe (972h)
PREY

MAN1

heh2, mug61, SPAC14C4.05c
LEM domain protein Man1, Sad1 interacting factor (predicted)
Schizosaccharomyces pombe (972h)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Control of heterochromatin localization and silencing by the nuclear membrane protein Lem2.

Barrales RR, Forn M, Georgescu PR, Sarkadi Z, Braun S

Transcriptionally silent chromatin localizes to the nuclear periphery, which provides a special microenvironment for gene repression. A variety of nuclear membrane proteins interact with repressed chromatin, yet the functional role of these interactions remains poorly understood. Here, we show that, in Schizosaccharomyces pombe, the nuclear membrane protein Lem2 associates with chromatin and mediates silencing and heterochromatin localization. Unexpectedly, we found ... [more]

Genes Dev. Jan. 15, 2016; 30(2);133-48 [Pubmed: 26744419]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: silencing (APO:0000046)

Additional Notes

  • mono-phenotypic sub-suppressing

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
LEM2 MAN1
Phenotypic Enhancement
Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
559760
LEM2 MAN1
Synthetic Growth Defect
Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Low-BioGRID
559756
LEM2 MAN1
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
559758
MAN1 LEM2
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-

Curated By

  • BioGRID